RBRC06385

Information1

Image
BRC No.RBRC06385
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Dhx15<tm1Hijo>
Former Common nameDHX15 flox, B6-Dhx15 floxed
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2012. C57BL/6 background.
Strain descriptionDhx15 floxed mice. Exon 2 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesSci. Signal, 7(323), ra40, 2014. 24782566

Health Report

Examination Date / Room / Rack2015/10/05Room:3-5Rack:B
2015/08/03Room:3-5Rack:B
2015/06/01Room:3-5Rack:B
2015/04/06Room:3-5Rack:B
2015/02/02Room:3-5Rack:B
2014/12/01Room:3-5Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dhx15DEAH (Asp-Glu-Ala-His) box polypeptide 155Dhx15<tm1Hijo>targeted mutation 1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Information2

Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse Dhx15 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHidenori Ichijo (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data