Strain Data Sheet

RBRC06364

Strain Information

Image
BRC No.RBRC06364
TypeChemically-induced Mutation
SpeciesMus musculus
Strain nameB6(D2)-Disc1<Rgsc1390>/Rod
Former Common nameDISC1 RGSC1390 - DISC1-L100P, B6-Disc1/1390
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentOriginated from RIKEN ENU-base gene-driven mutagenesis. Established the B6 congenic strains by Tatiana Lipina and John Roder, Mount Sinai Hospital. The mutant mice were backcrossed to C57BL/6 over 15 times (C57BL/6J and C57BL/6NCrl mixed).
Strain descriptionDISC1 has been reported as a responsible gene for mental illness which was originally found in a large Scottish family with major mental illness such as depression and schizophrenia. Two independently derived ENU-induced mutant mice which have missense mutations in exon 2 of Disc1 gene were developed and analyzed jointly by RIKEN (Japan), University of Edinburgh (UK), and Mount Sinai Hospital (Canada). The mutant mouse with Q31L (Disc1<Rgsc1393>) showed depressive-like behavior with defects, e.g., in the forced swim test. The other mutant mouse with L100P (Disc1<Rgsc1390>) exhibited schizophrenic-like behavior, with profound defect in prepulse inhibition and latent inhibition. These Disc1 missense mutant mice are expected to be very useful as models for depression and schizophrenia. Disc1<Rgsc1390> (L100P)(RBRC06364), Disc1<Rgsc1393> (Q31L)(RBRC06365).
Colony maintenanceHomozygote x Homozygote
References
Behavioral phenotypes of Disc1 missense mutations in mice.
Clapcote S J, Lipina T V, Millar J K, Mackie S, Christie S, Ogawa F, Lerch J P, Trimble K, Uchiyama M, Sakuraba Y, Kaneda H, Shiroishi T, Houslay M D, Henkelman R M, Sled J G, Gondo Y, Porteous D J, Roder J C
Neuron, 54, 387-402 (2007). 17481393

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Disc1disrupted in schizophrenia 18Disc1RIKEN Genomic Sciences Center (GSC), 1390
  • schizophrenia 9(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal entorhinal cortex morphology(MP:0006300)

  • abnormal latent inhibition of conditioning behavior(MP:0006299)

  • abnormal prepulse inhibition(MP:0003088)

  • abnormal spatial learning(MP:0001463)

  • abnormal spatial working memory(MP:0008428)
    • more 8 phenotypes
  • abnormal thalamus morphology(MP:0000832)

  • behavior/neurological phenotype(MP:0005386)

  • decreased brain size(MP:0000774)

  • decreased startle reflex(MP:0001489)

  • increased exploration in new environment(MP:0001415)

  • small cerebellum(MP:0000852)

  • small thalamus(MP:0020527)

  • thin cerebral cortex(MP:0006254)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationMouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 54, 387-402 (2007).The RECIPIENT of for-profit institution must contact the DEPOSITOR separately.
    The RECIPIENT of not for-profit institution must contact and discuss in good faith with the DEPOSITOR regarding inventorship, ownership, patent filing and licese fees and others, prior to filing a patent or make a contract with license fees with a third party for invention or products obtained from research results by using the BIOLOGICAL RESOURCES.
    DepositorRIKEN GSC (RIKEN GSC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data