Strain Data Sheet

RBRC06357

Strain Information

Image
BRC No.RBRC06357
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Msr1<tm1(HBEGF)Mtka>
Former Common nameCD204-DTR, B6-Msr1/DTR KI
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masato Tanaka, RIKEN Research Center for Allergy and Immunology in 2011. B6JN/1 ES cells were used. C57BL/6 background.
Strain descriptionCD204 (Msr1) DTR knock-in mice. HB-EGF is expressed from CD204 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
CD204-positive monocytes and macrophages ameliorate septic shock by suppressing proinflammatory cytokine production in mice.
Uchida Y, Nishitai G, Kikuchi K, Shibuya T, Asano K, Tanaka M
Biochem. Biophys. Rep., 23:100791 (2020). 32793817

CD204-positive monocytes and macrophages ameliorate septic shock by suppressing proinflammatory cytokine production in mice.
Yoshimi Uchida, Gen Nishitai, Kenta Kikuchi, Takumi Shibuya, Kenichi Asano, Masato Tanaka
Biochem. Biophys. Rep., 23:100791 (2020). 32793817

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site8Frt
  • Barrett esophagus(MedGEN)
    • HBB2rabbit beta-globin polyA8HBB2
  • Barrett esophagus(MedGEN)
    • HBEGFheparin-binding EGF-like growth factor (human)8HBEGF
  • Barrett esophagus(MedGEN)
    • Msr1macrophage scavenger receptor 18Msr1targeted mutation 1, Masato Tanaka
  • Barrett esophagus(MedGEN)
    • gb2phage T7 gb2 promoter(synthetic)8gb2
  • Barrett esophagus(MedGEN)
    • neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)
  • Barrett esophagus(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAhuman HB-EGF gene cDNA, Rabbit beta-globin polyA, Saccharomyces cerevisiae FRT sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistance gene, mouse Msr1 genomic DNA
    Research applicationFLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Rep., 23:100791 (2020). The recipient shall not distribute the biological resource to others without a written consent from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER (Masato Tanaka and Gen Nishitai), and then include them as co-authors in any publications resulted by the use of the BIOLOGICAL RESOURCE.
    DepositorMasato Tanaka (Tokyo University of Pharmacy and Life Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data