Strain Data Sheet


Strain Information

BRC No.RBRC06355
SpeciesMus musculus
Strain nameB6.Cg-Tg(Nes-EGFP)1Yamm
Former Common nameNestin-EGFP, B6-Nes-GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Masahiro Yamaguchi, PRESTO, Japan Science and Technology Corporation in 1998. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 and DBA/2 mixed background.
Strain descriptionNestin-GFP reporter mice, expressing a EFGP gene under the control of rat nestin promoter.
Colony maintenanceCarrier x Carrier
ReferencesNeuroReport, 11, 1991-1996 (2000). 10884058

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPrat Nestin promoter

Ordering Information

Donor DNARat nestin genomic DNA, jellyfish EGFP cDNA, rabbit beta globin polyA
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. NeuroReport, 11, 1991-1996 (2000).
DepositorMasahiro Yamaguchi (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Sawada R, Nakano-Doi A, Matsuyama T, Nakagomi N, Nakagomi T.
CD44 expression in stem cells and niche microglia/macrophages following ischemic stroke.
Stem Cell Investig 7 4(2020) 32309418