RBRC06355

Information1

Image
BRC No.RBRC06355
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Nes-EGFP)1Yamm
Former Common nameNestin-EGFP, B6-Nes-GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masahiro Yamaguchi, PRESTO, Japan Science and Technology Corporation in 1998. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 and DBA/2 mixed background.
Strain descriptionNestin-GFP reporter mice, expressing a EFGP gene under the control of rat nestin promoter.
Colony maintenanceCarrier x Carrier
ReferencesNeuroReport, 11, 1991-1996 (2000). 10884058

Health Report

Examination Date / Room / Rack2020/06/01Room:3-DRack:C
2020/02/17Room:3-DRack:C
2019/12/16Room:3-DRack:C
2019/10/21Room:3-DRack:C
2019/08/26Room:3-DRack:C
2019/06/17Room:3-DRack:C
2019/04/15Room:3-DRack:C
2019/02/18Room:3-DRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFPrat Nestin promoter

Information2

Donor DNARat nestin genomic DNA, jellyfish EGFP cDNA, rabbit beta globin polyA
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. NeuroReport, 11, 1991-1996 (2000).
DepositorMasahiro Yamaguchi (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data