RBRC06352

Information1

Image
BRC No.RBRC06352
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.129S4-Asf1a<Gt(S7-4B1)Sor>/Skmz
Former Common nameB6-CIA-I, B6-Asf1a Gt
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kazuhisa Sekimizu, Graduate School of Pharmaceutical Sciences in 2007. CIA-II (RBRC06350): Originated from Asf1b<Gt(ROSABetageo)437Muv> gene trapped mice (EMMA ID:01154). Backcrossed to C57BL/6. CIA-I (RBRC06351 and RBRC06352): Generated by using Asf1a<Gt(S7-4B1)Sor> gene trapped ES cells (MMRRC #13934-USD/S7-4B1). The mutant mice were backcrossed to ICR and C57BL/6 mice, respectively.
Strain descriptionCIA-II(Asf1b) gene trapped mice (B6 background)(RBRC06350), CIA-I (Asf1a) gene trapped mice (ICR mixed background: RBRC06351 and B6 background: RBRC06352). Homozygous and heterozygous mutant mice for CIA-II gene show no obvious abnormality. Homozygous mutant mice for CIA-I of ICR mixed and C57BL/6 background were perinatal and embryonic (around E12.5) lethal, respectively.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesNat. Genet., 36, 304-312 (2004). 14981515
Genes Dev., 14, 1332-1342 (2000). 10837026
Genes Dev., 5, 1513-1523 (1991). 1653172

Health Report

Examination Date / Room / Rack2015/03/23Room:4-ARack:F
2015/01/19Room:4-ARack:F
2014/11/17Room:4-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Asf1aanti-silencing function 1A histone chaperone10Asf1a<Gt(S7-4B1)Sor>gene trap S7-4B1, Philippe Soriano

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)10GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hyghygromycin phasphotransferase (E. coli)10Hygmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
LTRretrovirus LTR (viral long terminal repeat)10LTR

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor (adenovirus)10SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SDadenovirus splice donor (SD)10SD

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)10

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10

Information2

Donor DNAadenovirus splice acceptor (SA), E. coli beta-galactosidase, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation signal, yeast Frt sites, mouse phosphoglycerate kinase promoter, E. coli hygromycin resistance gene, adenovirus splice donor (SD), retroviral LTR, mouse Asf1b genomic DNA
Research applicationDevelopmental Biology Research
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。 (will be announced).The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a mention of the Sanger Institute Gene Trap Resource as the source of the original allele are requested.
DepositorKazuhisa Sekimizu (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data