Strain Data Sheet

RBRC06351

Strain Information

Image
BRC No.RBRC06351
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameICR;129S4(B6)-Asf1a<Gt(S7-4B1)Sor>/Skmz
Former Common nameICR-CIA-I, ICR-Asf1a Gt
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kazuhisa Sekimizu, Graduate School of Pharmaceutical Sciences in 2007. CIA-II (RBRC06350): Originated from Asf1b<Gt(ROSABetageo)437Muv> gene trapped mice (EMMA ID:01154). Backcrossed to C57BL/6. CIA-I (RBRC06351 and RBRC06352): Generated by using Asf1a<Gt(S7-4B1)Sor> gene trapped ES cells (MMRRC #13934-USD/S7-4B1). The mutant mice were backcrossed to ICR and C57BL/6 mice, respectively.
Strain descriptionCIA-II(Asf1b) gene trapped mice (B6 background)(RBRC06350), CIA-I (Asf1a) gene trapped mice (ICR mixed background: RBRC06351 and B6 background: RBRC06352). Homozygous and heterozygous mutant mice for CIA-II gene show no obvious abnormality. Homozygous mutant mice for CIA-I of ICR mixed and C57BL/6 background were perinatal and embryonic (around E12.5) lethal, respectively.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:ICR]
References
E-MAP-115, encoding a microtubule-associated protein, is a retinoic acid-inducible gene required for spermatogenesis.
Komada M, McLean D J, Griswold M D, Russell L D, Soriano P
Genes Dev., 14, 1332-1342 (2000). 10837026

Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis.
Chen W V, Delrow J, Corrin P D, Frazier J P, Soriano P
Nat. Genet., 36, 304-312 (2004). 14981515

Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Friedrich G, Soriano P
Genes Dev., 5, 1513-1523 (1991). 1653172

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Asf1aanti-silencing function 1A histone chaperone10Asf1agene trap S7-4B1, Philippe Soriano
Frtyeast FRT (flippase recombination target) site10Frt
Frtyeast FRT (flippase recombination target) site10Frt
GHGrowth hormone polyA (Bovine)10GH
Hyghygromycin phasphotransferase (E. coli)10Hygmouse phosphoglycerate kinase promoter (PGK promoter)
LTRretrovirus LTR (viral long terminal repeat)10LTR
SASplice acceptor (adenovirus)10SA
SDadenovirus splice donor (SD)10SD
lacZbeta-galactosidase (E. coli)10
neoneomycin resistance gene (E. coli)10

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus splice acceptor (SA), E. coli beta-galactosidase, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation signal, yeast Frt sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli hygromycin resistance gene, adenoviral splice donor (SD), retrovial LTR, mouse Asf1a genomic DNA
    Research applicationDevelopmental Biology Research
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a mention of the Sanger Institute Gene Trap Resource as the source of the original allele are requested.
    DepositorKazuhisa Sekimizu (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data