Strain Data Sheet

RBRC06350

Strain Information

Image
BRC No.RBRC06350
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.129S2-Asf1b<Gt(ROSABetageo)437Muv>/Skmz
Former Common nameB6-CIA-II, B6-Asf1b Gt
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kazuhisa Sekimizu, Graduate School of Pharmaceutical Sciences in 2007. CIA-II (RBRC06350): Originated from Asf1b<Gt(ROSABetageo)437Muv> gene trapped mice (EMMA ID:01154). Backcrossed to C57BL/6. CIA-I (RBRC06351 and RBRC06352): Generated by using Asf1a<Gt(S7-4B1)Sor> gene trapped ES cells (MMRRC #13934-USD/S7-4B1). The mutant mice were backcrossed to ICR and C57BL/6 mice, respectively.
Strain descriptionCIA-II(Asf1b) gene trapped mice (B6 background)(RBRC06350), CIA-I (Asf1a) gene trapped mice (ICR mixed background: RBRC06351 and B6 background: RBRC06352). Homozygous and heterozygous mutant mice for CIA-II gene show no obvious abnormality. Homozygous mutant mice for CIA-I of ICR mixed and C57BL/6 background were perinatal and embryonic (around E12.5) lethal, respectively.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Friedrich G, Soriano P
Genes Dev., 5, 1513-1523 (1991). 1653172

E-MAP-115, encoding a microtubule-associated protein, is a retinoic acid-inducible gene required for spermatogenesis.
Komada M, McLean D J, Griswold M D, Russell L D, Soriano P
Genes Dev., 14, 1332-1342 (2000). 10837026

Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis.
Chen W V, Delrow J, Corrin P D, Frazier J P, Soriano P
Nat. Genet., 36, 304-312 (2004). 14981515

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Asf1banti-silencing function 1B histone chaperone8Asf1bgene trap 437, Muriel Vernet
GHGrowth hormone polyA (Bovine)8GH
LTRretrovirus LTR (viral long terminal repeat)8LTR
SASplice acceptor (adenovirus)8SA
lacZbeta-galactosidase (E. coli)8
neoneomycin resistance gene (E. coli)8

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus splice acceptor (SA), E. coli beta-galactosidase, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation signal, retrovial LTR, mouse Asf1a genomic DNA
    Research applicationCancer Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.)
    DepositorKazuhisa Sekimizu (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data