Strain Data Sheet

RBRC06348

Strain Information

Image
BRC No.RBRC06348
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Cck<tm2b(EUCOMM)Hmgu>/B06
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyadenylation site9
Cckcholecystokinin9Ccktargeted mutation 2b, Helmholtz Zentrum Muenchen GmbH
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)9En2
Frtyeast FRT (flippase recombination target) site9Frt
Iresinternal ribosomal entry site (EMCV)9
lacZbeta-galactosidase (E. coli)9
loxPphage P1 loxP9loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal kidney morphology(MP:0002135)

  • increased startle reflex(MP:0001488)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) site, En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, Bacteriophage P1 loxP site, mouse Cck genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data