RBRC06346

Information1

Image
BRC No.RBRC06346
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Acsm4<tm1(cre)Hsak>
Former Common nameO-MACS-Cre, Acsm4/cre KI
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Yuichiro Oka, Graduate School of Science, The University of Tokyo in 2004. D3 ES cells were used to generate the mutant mice. IICR mixed background.
Strain descriptionOMACS (Acsm4) cre (iCre) knock-in mice.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Crlj:CD1(ICR)]
ReferencesNature, 450, 503-508 (2007). 17989651

Health Report

Examination Date / Room / Rack2016/09/26Room:4-BRack:L
2016/07/25Room:4-BRack:L
2016/06/22Room:4-BRack:L
2016/05/30Room:4-BRack:L
2016/03/28Room:4-BRack:L
2016/01/25Room:4-BRack:L
2015/11/30Room:4-BRack:L
2015/09/28Room:4-BRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Acsm4acyl-CoA synthetase medium-chain family member 47Acsm4<tm1.1(cre)Hsak>targeted mutation 1.1, Hitoshi Sakano

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
iCrephage P1 codon improved Cre (iCre)7iCre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal7

Information2

Donor DNAphage P1 cre recombinase, SV40 polyA signal, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter, E.coli neo, mouse Acsm4 (olfactory specific medium-chain acyl CoA synthetase) genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 450, 503-508 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorHitoshi Sakano (University of Fukui)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data