Strain Data Sheet

RBRC06345

Strain Information

Image
BRC No.RBRC06345
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ube2g1<tm1b(EUCOMM)Hmgu>/B02
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ube2g1ubiquitin-conjugating enzyme E2G 111Ube2g1targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal pelvic girdle bone morphology(MP:0004509)

  • cardiovascular system phenotype(MP:0005385)

  • decreased Ly6C-positive mature NK cell number(MP:0013675)

  • decreased effector memory CD8-positive, alpha-beta T cell number(MP:0010851)

  • increased CD11b-high dendritic cell number(MP:0013648)
    • more 6 phenotypes
  • increased NK cell number(MP:0008044)

  • increased dendritic cell number(MP:0008126)

  • increased or absent threshold for auditory brainstem response(MP:0011967)

  • male infertility(MP:0001925)

  • short tibia(MP:0002764)

  • small liver(MP:0000601)
    • Detailed phenotype dataPhenopub Link

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP site, mouse Ube2g1 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data