BRC No.RBRC06344
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-App<tm3(NL-G-F)Tcs>
Former Common nameAPP<NL-G-F>, B6-App/NL-G-F KI
H-2 Haplotype
ES Cell lineiTL IC1 [C57BL/6NTac]
Background strain
Strain developmentDeveloped by Takashi Saito and Takaomi C. Saido, RIKEN Brain Science Institute in 2009. C57BL/6 derived ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with EIIa-cre mice (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J). C57BL/6 background.
Strain descriptionApp knock-in mice having Swedish mutation (NL) (RBRC06342), Swedish/Iberian (NL-F) mutations (RBRC06343) and Swedish/Iberian/Arctic mutations (NL-G-F) (RBRC06344). Homozygous mutant mice are viable and fertile.
Colony maintenanceHeterozygote x Heterozygote, Wild-type [or Crossing to C57BL/6J(Crlj)]
ReferencesEMBO J., 36, 2473-2487 (2017). 28768718
Nature Neuroscience, 17, 661-663 (2014). 24728269

Health Report

Examination Date / Room / Rack2021/02/01Room:3-ARack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Appamyloid beta (A4) precursor protein16App<tm3(NL-G-F)Tcs>targeted mutation 3, Takaomi C. Saido

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP


Donor DNAphage P1 loxP site, yeast FRT (flipase recombination target) site, mouse App genomic DNA (mutant type)
Research applicationCre/loxP system
FLP/frt system
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RIKEN BRC does not provide any for-profit organization with the BIOLOGICAL RESOURCE. For-profit organizations must contact the DEPOSITOR (saido@brain.riken.jp) for a licensing contract. RECIPIENT shall supply DEPOSITOR with a written report about all research results related to the BIOLOGICAL RESOURCE as outlined in the Research Plan. RECIPIENT will have the right to publish and disclose the results of the research and will submit the intended publication to DEPOSITOR at least thirty (30) days prior to submission of the abstracts and manuscripts. DEPOSITOR may within this 30-day period request RECIPIENT, in writing, to delete any reference to DEPOSITOR's CONFIDENTIAL information. If any invention is conceived and reduced to practice by RECIPIENT in the performance of the Research Plan involving the BIOLOGICAL RESOURCE during the term of this MTA, RECIPIENT agrees to promptly inform the invention to DEPOSITOR and the parties will consult each other to determine inventorship and ownership of the invention based on the respective parties contribution to the invention, before filing an application for a patent. In the case of a jointly owned application, the parties will separately enter into a joint application agreement including the sharing of ownership, patent costs and licensing income, as well as the responsible party for the application procedures etc. RECIPIENT agrees to acknowledge Hiroki SASAGURI, Takashi SAITO and Takaomi C. SAIDO, according to generally accepted authorship attributions, as co-authors in the first domestic oral presentation, first international oral presentation, and first written international publication as related to the BIOLOGICAL RESOURCE unless otherwise requested by the DEPOSITOR. The purpose of co-authorship is not for us to increase our number of publications but rather to confirm compliance with the guidelines on nomenclature, correct use of antibodies, etc. and to discuss the relevance of the observed results in depth. Thereafter, RECIPIENT agrees to acknowledge Hiroki SASAGURI, Takashi SAITO and Takaomi C. SAIDO in an acknowledgement section, and does not need to co-author Hiroki SASAGURI, Takashi SAITO and Takaomi C. SAIDO. RECIPIENT also agrees to cite Nature Neuroscience 17, 661-663 (2014) and EMBO Journal 36, 2473-2487 (2017) in all publications obtained using BIOLOGICAL RESOURCE. RECIPIENT agrees to acknowledge DEPOSITOR in all oral presentations and written publications as related to the BIOLOGICAL RESOURCE. RECIPIENT shall keep confidential any or all information marked CONFIDENTIAL that is received from DEPOSITOR and related to the BIOLOGICAL RESOURCE including oral discussions if they are reduced to writing within thirty (30) days and are marked "CONFIDENTIAL". The BIOLOGICAL RESOURCE is the property of the RIKEN (The Institute of Physical and Chemical Research).
DepositorTakaomi C. Saido (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Sep 2014
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. MTA (English), MTA (Japanese),
  3. APPROVAL FORM (English), APPROVAL FORM (Japanese)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data