Strain Data Sheet

RBRC06344

Strain Information

Image
BRC No.RBRC06344
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-App<tm3(NL-G-F)Tcs>
Former Common nameAPP<NL-G-F>, B6-App/NL-G-F KI
H-2 Haplotype
ES Cell lineiTL IC1 [C57BL/6NTac]
Background strain
Appearance
Strain developmentDeveloped by Takashi Saito and Takaomi C. Saido, RIKEN Brain Science Institute in 2009. C57BL/6 derived ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with EIIa-cre mice (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J). C57BL/6 background.
Strain descriptionApp knock-in mice having Swedish mutation (NL) (RBRC06342), Swedish/Iberian (NL-F) mutations (RBRC06343) and Swedish/Iberian/Arctic mutations (NL-G-F) (RBRC06344). Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crl)]
ReferencesFront. Neurosci., 16:807473 (2022). 35431779
Nat. Neurosci., 17(5):661-663 (2014). 24728269

Health Report

Examination Date / Room / Rack2024/04/08Room:3-ARack:A
2024/01/09Room:3-ARack:D
2023/10/10Room:3-ARack:A and D
2023/07/10Room:3-ARack:D
2023/04/10Room:3-ARack:D
2023/02/13Room:3-ARack:D
2023/01/10Room:3-ARack:D
2022/12/05Room:3-ARack:D
2022/10/11Room:3-ARack:D
2022/08/31Room:3-ARack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Appamyloid beta (A4) precursor protein16App<tm3(NL-G-F)Tcs>targeted mutation 3, Takaomi C. Saido

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Ordering Information

Donor DNAphage P1 loxP site, yeast FRT (flipase recombination target) site, mouse App genomic DNA (mutant type)
Research applicationCre/loxP system
FLP/frt system
Mouse Models for Human Disease
Specific Term and ConditionsThe BIOLOGICAL RESOURCE is the property of the RIKEN (The Institute of Physical and Chemical Research). RECIPIENT agrees to acknowledge Takashi SAITO and Takaomi C. SAIDO in an acknowledgement section. RECIPIENT also agrees to cite Nature Neuroscience 17, 661-663 (2014) in all publications obtained using BIOLOGICAL RESOURCE. RECIPIENT agrees to acknowledge DEPOSITOR in all oral presentations and written publications as related to the BIOLOGICAL RESOURCE. RECIPIENT shall keep confidential any or all information marked CONFIDENTIAL that is received from DEPOSITOR and related to the BIOLOGICAL RESOURCE including oral discussions if they are reduced to writing within thirty (30) days and are marked "CONFIDENTIAL". For-profit organizations must contact the DEPOSITOR (takaomi.saido@riken.jp) for a licensing contract. If any invention is conceived and reduced to practice by RECIPIENT in the performance of the Research Plan involving the BIOLOGICAL RESOURCE during the term of this MTA, RECIPIENT agrees to promptly inform the invention to DEPOSITOR and the parties will consult each other to determine inventorship and ownership of the invention based on the respective parties contribution to the invention, before filing an application for a patent. In the case of a jointly owned application, the parties will separately enter into a joint application agreement including the sharing of ownership, patent costs and licensing income, as well as the responsible party for the application procedures etc.
DepositorTakaomi C. Saido (RIKEN CBS)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese/English)
Genotyping protocol -PCR-
Mouse of the Month Sep 2014

BRC mice in Publications

Nagashima K, Watanabe H, Akasaka T, Ono M.
Development of Triphenylmethane Dyes for In Vivo Fluorescence Imaging of Aβ Oligomers.
ACS Chem Neurosci 15(11) 2233-2242(2024) 38753435

Xie Z, Meng J, Kong W, Wu Z, Lan F, Narengaowa, Hayashi Y, Yang Q, Bai Z, Nakanishi H, Qing H, Ni J.
Microglial cathepsin E plays a role in neuroinflammation and amyloid β production in Alzheimer's disease.
Aging Cell 21(3) e13565(2022) 35181976

Takebayashi Y, Yamazaki Y, Yamada H, Yazawa K, Nakamori M, Kurashige T, Morino H, Takahashi T, Sotomaru Y, Maruyama H.
Apolipoprotein E genotype-dependent accumulation of amyloid β in APP-knock-in mouse model of Alzheimer's disease.
Biochem Biophys Res Commun 683 149106(2023) 37857162

Yoshiko Takebayashi, Yu Yamazaki, Hidetada Yamada, Kyosuke Yazawa, Masahiro Nakamori, Takashi Kurashige, Hiroyuki Morino, Tetsuya Takahashi, Yusuke Sotomaru, Hirofumi Maruyama
Apolipoprotein E genotype-dependent accumulation of amyloid β in APP-knock-in mouse model of Alzheimer's disease
Biochemical and Biophysical Research Communications (2023)

Ho H, Kejzar N, Sasaguri H, Saito T, Saido TC, De Strooper B, Bauza M, Krupic J.
A fully automated home cage for long-term continuous phenotyping of mouse cognition and behavior.
Cell Rep Methods 3(7) 100532(2023) 37533650

Tomoyuki Mano, Ken Murata, Kazuhiro Kon, Chika Shimizu, Hiroaki Ono, Shoi Shi, Rikuhiro G. Yamada, Kazunari Miyamichi, Etsuo A. Susaki, Kazushige Touhara, Hiroki R. Ueda
CUBIC-Cloud provides an integrative computational framework toward community-driven whole-mouse-brain mapping
Cell Reports Methods 1 100038(2021) 35475238

Liu X, Zhou Q, Zhang JH, Wang KY, Saito T, Saido TC, Wang X, Gao X, Azuma K.
Microglia-Based Sex-Biased Neuropathology in Early-Stage Alzheimer's Disease Model Mice and the Potential Pharmacologic Efficacy of Dioscin.
Cells 10(11) (2021) 34831483

Yoshida K, Kato D, Sugio S, Takeda I, Wake H.
Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer's disease.
Front Cell Neurosci 17 1154196(2023) 38026691

Hiroki Sasaguri, Shoko Hashimoto, Naoto Watamura, Kaori Sato, Risa Takamura, Kenichi Nagata, Satoshi Tsubuki, Toshio Ohshima, Atsushi Yoshiki, Kenya Sato, Wakako Kumita, Erika Sasaki, Shinobu Kitazume, Per Nilsson, Bengt Winblad, Takashi Saito, Nobuhisa Iwata, Takaomi C. Saido
Recent Advances in the Modeling of Alzheimer’s Disease
Frontiers Neuroscience 16 (2022) 35431779

Kaneko R, Matsui A, Watanabe M, Harada Y, Kanamori M, Awata N, Kawazoe M, Takao T, Kobayashi Y, Kikutake C, Suyama M, Saito T, Saido TC, Ito M.
Increased neutrophils in inflammatory bowel disease accelerate the accumulation of amyloid plaques in the mouse model of Alzheimer's disease.
Inflamm Regen 43(1) 20(2023) 36922861

Abdelhamid M, Zhou C, Ohno K, Kuhara T, Taslima F, Abdullah M, Jung CG, Michikawa M.
Probiotic Bifidobacterium breve Prevents Memory Impairment Through the Reduction of Both Amyloid-β Production and Microglia Activation in APP Knock-In Mouse.
J Alzheimers Dis 85(4) 1555-1571(2022) 34958017

Akasaka T, Watanabe H, Ono M.
In Vivo Near-Infrared Fluorescence Imaging Selective for Soluble Amyloid β Aggregates Using y-Shaped BODIPY Derivative.
J Med Chem (2023) 37824378

Murakami A, Hara T, Yamada-Kubota C, Kuwahara M, Ichikawa T, Minagi S.
Lack of occlusal support did not impact amyloid β deposition in APP knock-in mice.
J Prosthodont Res 66(1) 161-166(2022) 34305086

Minamisawa M, Sato Y, Ishiguro E, Taniai T, Sakamoto T, Kawai G, Saito T, Saido TC.
Amelioration of Alzheimer's Disease by Gut-Pancreas-Liver-Brain Interaction in an App Knock-In Mouse Model.
Life (Basel) 12(1) (2021) 35054427

Honda K, Saito Y, Saito H, Toyoda M, Abe R, Saito T, Saido TC, Michikawa M, Taru H, Sobu Y, Hata S, Nakaya T, Suzuki T.
Accumulation of amyloid-β in the brain of mouse models of Alzheimer's disease is modified by altered gene expression in the presence of human apoE isoforms during aging.
Neurobiol Aging 123 63-74(2023) 36638682

Ye Q, Gast G, Su X, Saito T, Saido TC, Holmes TC, Xu X.
Hippocampal neural circuit connectivity alterations in an Alzheimer's disease mouse model revealed by monosynaptic rabies virus tracing.
Neurobiol Dis 172 105820(2022) 35843448

Mona Abdelhamid, Cha-Gyun Jung, Chunyu Zhou, Rieko Inoue, Yuxin Chen, Yoshiki Sento, Hideki Hida, Makoto Michikawa
Potential Therapeutic Effects of Bifidobacterium breve MCC1274 on Alzheimer’s Disease Pathologies in AppNL-G-F Mice
Nutrients 16 538(2024)

Fukuda M, Okanishi H, Ino D, Ono K, Kawamura S, Wakai E, Miyoshi T, Sato T, Ohta Y, Saito T, Saido TC, Inohara H, Kanai Y, Hibino H.
Disturbance in the protein landscape of cochlear perilymph in an Alzheimer's disease mouse model.
PLoS One 19(5) e0303375(2024) 38728348

Bellio TA, Laguna-Torres JY, Campion MS, Chou J, Yee S, Blusztajn JK, Mellott TJ.
Perinatal choline supplementation prevents learning and memory deficits and reduces brain amyloid Aβ42 deposition in AppNL-G-F Alzheimer's disease model mice.
PLoS One 19(2) e0297289(2024) 38315685

Yamada H, Yamazaki Y, Takebayashi Y, Yazawa K, Sasanishi M, Motoda A, Nakamori M, Morino H, Takahashi T, Maruyama H.
The long-term effects of heated tobacco product exposure on the central nervous system in a mouse model of prodromal Alzheimer's disease.
Sci Rep 14(1) 227(2024) 38167640

Gannon M, Wang B, Stringfellow SA, Quintin S, Mendoza I, Srikantha T, Roberts AC, Saito T, Saido TC, Roberson ED, Yacoubian TA.
14-3-3θ Does Not Protect against Behavioral or Pathological Deficits in Alzheimer's Disease Mouse Models.
eNeuro 9(3) (2022) 35697511