RBRC06336

Information1

Image
BRC No.RBRC06336
TypeTargeted Mutation and Spontaneous Mutation CongenicCartagena
SpeciesMus musculus
Strain nameNOD.Cg-Prkdc<scid> E2f1<tm1Meg>
Former Common nameNOD.e2f1<+/->, NOD-scid/E2f1 KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Hidenobu Senpuku, National Institute of Infectious Diseases. The E2f1<tm1Meg> mice of C57BL/6 and 129 mixed background were backcrossed to NOD/LtJ mice, and furthermore the mutant mice were backcrossed to NOD/SCID mice.
Strain descriptionE2f1 deficient NOD/SCID mice. Part of exon 3 to exon 4 of E2f1 gene was replaced with a neo cassette. E2f1 homozygous mutant mice are immunodeficiency and show decreased saliva production.
Colony maintenanceE2f1: Heterozygote x Wild-type; scid: Homozygote x Homozygote [or Crossing to NOD/ShiJic-scid/Jcl]
ReferencesPLoS ONE. 7: e32063. 22363797
J. Immunol., 173, 4908-4918 (2004). 15470032
Exp. Biol. Med. (Maywood)., 234, 1525-1536 (2009). 19934373
Cell, 85, 549-561 (1996). 8653790

Health Report

Examination Date / Room / Rack2018/05/14Room:2-検Rack:F
2018/03/12Room:2-検Rack:F
2018/01/15Room:2-検Rack:F
2017/11/13Room:2-検Rack:F
2017/09/11Room:2-検Rack:F
2017/07/10Room:2-検Rack:F
2017/05/15Room:2-検Rack:F
2017/03/13Room:2-検Rack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Prkdcprotein kinase, DNA activated, catalytic polypeptide16Prkdc<scid>severe combined immunodeficiency

Gene symbolGene nameChr.Allele symbolAllele namePromoter
E2f1E2F transcription factor 12E2f1<tm1Meg>targeted mutation 1, Michael E Greenburg

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse E2f1 genomic DNA
Research applicationImmunology and Inflammation Research
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Biol. Med. (Maywood)., 234, 1525-1536 (2009).
DepositorHidenori Senpuku (National Institute of Infectious Diseases)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data