RBRC06331

Information1

Image
BRC No.RBRC06331
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129-Dtx4<Gt(431E3)Cmhd>
Former Common nameDtx4 KO, B6-Dtx4 Gt
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Naifang Lu, Athanasia Spandidos and Brian Seed, Massachusetts General Hospital. The gene trapped mice were generated by using a CMHD-GT_431-E3-3 ES clone from Canadian Mouse Mutant Repository. C57BL/6 and 129 mixed background.
Strain descriptionDtx4 gene trapped mice (originated from Canadian Mouse Mutant Repository, Vector: pNMDi4, CMHD-GT_431-E3-3 clone. Homozygous mutant mice show no obvious abnormality. Under investigation (details unknown).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
References

Health Report

Examination Date / Room / Rack2015/06/15Room:3-ARack:G
2015/04/20Room:3-ARack:G
2015/02/16Room:3-ARack:G
2014/12/15Room:3-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dtx4deltex 4 homolog (Drosophila)19Dtx4<Gt(431E3)Cmhd>gene trap 431E3, Centre for Modeling Human Disease

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)19GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hprtmouse Hprt splice donor (SD)19Hprt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SAhuman adenovirus type 3/5 SA (splice acceptor)19SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)19Venus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAHuman adenovirus type 3/5 SA (splice acceptor), flexible linker (FL), jellyfish Venus, P1 phage loxP, Eukaryotic translation initiation factor 3 subunit 7, BGH polyA, mouse PGK promoter, E. coli neomycin resistance gene, mouse Hprt SD (splice donor)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorBrian Seed (Massachusetts General Hospital)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data