Strain Data Sheet

RBRC06330

Strain Information

Image
BRC No.RBRC06330
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Ly6a-KDM1A*v2)6Fscr
Former Common nameLSD1 Tg#6, B6-Ly6A-KDM1A/v2 Tg#6
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Taeko Wada, Center for Molecular Medicine, Jichi Medical University in 2012. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. Line 3 (RBRC06329), Line 6 (R BRC06330).
Strain descriptionLy6A-KDM1A transgenic mice. These mice express the human KDM1A (LSD1) (transcript variant 2) under the control of the mouse Ly6A promoter. Tg mice show no obvious abnormality. Under investigation (details unknown).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
References
Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemia.
Schenk T, Chen W C, Gollner S, Howell L, Jin L, Hebestreit K, Klein H U, Popescu A C, Burnett A, Mills K, Casero R A Jr, Marton L, Woster P, Minden M D, Dugas M, Wang J C, Dick J E, Muller-Tidow C, Petrie K, Zelent A
Nat Med. 11, 605-611, (2012). 22406747

The histone demethylase KDM1A sustains the oncogenic potential of MLL-AF9 leukemia stem cells.
Harris W J, Huang X, Lynch J T, Spencer G J, Hitchin J R, Li Y, Ciceri F, Blaser J G, Greystoke B F, Jordan A M, Miller C J, Ogilvie D J, Somervaille T C
Cancer Cell. 21(4):473-487, U, (2012). 22464800

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
KDM1Alysine (K)-specific demethylase 1A (human)UNKDM1Amouse Sca-1(Ly6A) promoter
neoneomycin resistance gene (E. coli)UN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, Human LSD1(KDM1A)gene cDNA, mouse Sca-1(Ly6A) promoter, SV40 PolyA signal
    Research applicationCancer Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.)
    DepositorYusuke Furukawa (Jichi Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data