RBRC06328

Information1

Image
BRC No.RBRC06328
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6(C)-Tg(Col2a1-Ctgf,lacZ)05908-01-2Hatt
Former Common nameCTGF<Col2a1tg>, B6-Col2a1-Ctgf/lacZ Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takako Hattori and Masaharu Takigawa, Okayama University Graduate School of Medicine. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6 and BALB/c mixed background.
Strain descriptionCTGF transgenic mice. The mice express the CTGF/CCN2 (HA-tagged) and lacZ under the control of the Col2a1 promoter/enhancer (6-kb).
Colony maintenanceCarrier x Noncarrier [or Crossing to C57BL/6NCrSlc]
ReferencesPLoS One. 2013;8(3):e59226. 23555635
PLoS One. 2013 Aug 12;8(8):e71156. 23951098

Health Report

Examination Date / Room / Rack2014/09/08Room:2-検Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ctgfconnective tissue growth factorUNCtgfmouse Col2a1 promoter-enhancer

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor (adenovirus)UNSA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAmouse Col2a1 promoter-enhancer, adenovirus splice acceptor (SA), mouse Ctgf cDNA with HA tag cDNA, human influenza virus HA (hemagglutinin) tag sequence, EMCV Internal ribosomal enrty site (ires), E.coli lacZ, SV40 polyA signal
Research applicationCancer Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2013 8(3):e59226.  PLoS One. 2013 8(8):e71156.For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITORS and must obtain a prior written consent from the DEPOSITORS.  The RECIPIENT must contact the DEPOSITORS in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasaharu Takigawa (Okayama University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data