Strain Data Sheet

RBRC06328

Strain Information

Image
BRC No.RBRC06328
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6(C)-Tg(Col2a1-Ctgf,lacZ)05908-01-2Hatt
Former Common nameCTGF<Col2a1tg>, B6-Col2a1-Ctgf/lacZ Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takako Hattori and Masaharu Takigawa, Okayama University Graduate School of Medicine. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6 and BALB/c mixed background.
Strain descriptionCTGF transgenic mice. The mice express the CTGF/CCN2 (HA-tagged) and lacZ under the control of the Col2a1 promoter/enhancer (6-kb).
Colony maintenanceCarrier x Noncarrier [or Crossing to C57BL/6NCrSlc]
References
CCN family member 2/connective tissue growth factor (CCN2/CTGF) has anti-aging effects that protect articular cartilage from age-related degenerative changes.
Itoh S, Hattori T, Tomita N, Aoyama E, Yutani Y, Yamashiro T, Takigawa M
PLoS One. 2013 Aug 12;8(8):e71156. 23951098

Cartilage-specific over-expression of CCN family member 2/connective tissue growth factor (CCN2/CTGF) stimulates insulin-like growth factor expression and bone growth.
Tomita N, Hattori T, Itoh S, Aoyama E, Yao M, Yamashiro T, Takigawa M
PLoS One. 2013;8(3):e59226. 23555635

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
  • achondrogenesis type II(MedGEN)

  • avascular necrosis of femoral head, primary, 1(MedGEN)

  • Czech dysplasia, metatarsal type(MedGEN)
    • more 13 Diseases
  • Kniest dysplasia(DisGeNET, MedGEN)

  • Legg-Calve-Perthes disease(MedGEN)

  • mild spondyloepiphyseal dysplasia due to COL2A1 mutation with early-onset osteoarthritis(MedGEN)

  • multiple epiphyseal dysplasia, Beighton type(MedGEN)

  • platyspondylic dysplasia, Torrance type(MedGEN)

  • spondyloepimetaphyseal dysplasia, Strudwick type(MedGEN)

  • spondyloepiphyseal dysplasia congenita(MedGEN)

  • spondyloepiphyseal dysplasia, Stanescu type(MedGEN)

  • spondylometaphyseal dysplasia, 'corner fracture' type(MedGEN)

  • spondyloperipheral dysplasia(MedGEN)

  • Stickler syndrome type 1(MedGEN)

  • Stickler syndrome, type I, nonsyndromic ocular(MedGEN)

  • vitreoretinopathy with phalangeal epiphyseal dysplasia(MedGEN)
    • Ctgfconnective tissue growth factorUNCtgfmouse Col2a1 promoter-enhancer
  • achondrogenesis type II(MedGEN)

  • avascular necrosis of femoral head, primary, 1(MedGEN)

  • Czech dysplasia, metatarsal type(MedGEN)
    • more 13 Diseases
  • Kniest dysplasia(DisGeNET, MedGEN)

  • Legg-Calve-Perthes disease(MedGEN)

  • mild spondyloepiphyseal dysplasia due to COL2A1 mutation with early-onset osteoarthritis(MedGEN)

  • multiple epiphyseal dysplasia, Beighton type(MedGEN)

  • platyspondylic dysplasia, Torrance type(MedGEN)

  • spondyloepimetaphyseal dysplasia, Strudwick type(MedGEN)

  • spondyloepiphyseal dysplasia congenita(MedGEN)

  • spondyloepiphyseal dysplasia, Stanescu type(MedGEN)

  • spondylometaphyseal dysplasia, 'corner fracture' type(MedGEN)

  • spondyloperipheral dysplasia(MedGEN)

  • Stickler syndrome type 1(MedGEN)

  • Stickler syndrome, type I, nonsyndromic ocular(MedGEN)

  • vitreoretinopathy with phalangeal epiphyseal dysplasia(MedGEN)
    • HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA
  • achondrogenesis type II(MedGEN)

  • avascular necrosis of femoral head, primary, 1(MedGEN)

  • Czech dysplasia, metatarsal type(MedGEN)
    • more 13 Diseases
  • Kniest dysplasia(DisGeNET, MedGEN)

  • Legg-Calve-Perthes disease(MedGEN)

  • mild spondyloepiphyseal dysplasia due to COL2A1 mutation with early-onset osteoarthritis(MedGEN)

  • multiple epiphyseal dysplasia, Beighton type(MedGEN)

  • platyspondylic dysplasia, Torrance type(MedGEN)

  • spondyloepimetaphyseal dysplasia, Strudwick type(MedGEN)

  • spondyloepiphyseal dysplasia congenita(MedGEN)

  • spondyloepiphyseal dysplasia, Stanescu type(MedGEN)

  • spondylometaphyseal dysplasia, 'corner fracture' type(MedGEN)

  • spondyloperipheral dysplasia(MedGEN)

  • Stickler syndrome type 1(MedGEN)

  • Stickler syndrome, type I, nonsyndromic ocular(MedGEN)

  • vitreoretinopathy with phalangeal epiphyseal dysplasia(MedGEN)
    • IRESinternal ribosomal entry site (EMCV)UN
  • achondrogenesis type II(MedGEN)

  • avascular necrosis of femoral head, primary, 1(MedGEN)

  • Czech dysplasia, metatarsal type(MedGEN)
    • more 13 Diseases
  • Kniest dysplasia(DisGeNET, MedGEN)

  • Legg-Calve-Perthes disease(MedGEN)

  • mild spondyloepiphyseal dysplasia due to COL2A1 mutation with early-onset osteoarthritis(MedGEN)

  • multiple epiphyseal dysplasia, Beighton type(MedGEN)

  • platyspondylic dysplasia, Torrance type(MedGEN)

  • spondyloepimetaphyseal dysplasia, Strudwick type(MedGEN)

  • spondyloepiphyseal dysplasia congenita(MedGEN)

  • spondyloepiphyseal dysplasia, Stanescu type(MedGEN)

  • spondylometaphyseal dysplasia, 'corner fracture' type(MedGEN)

  • spondyloperipheral dysplasia(MedGEN)

  • Stickler syndrome type 1(MedGEN)

  • Stickler syndrome, type I, nonsyndromic ocular(MedGEN)

  • vitreoretinopathy with phalangeal epiphyseal dysplasia(MedGEN)
    • SASplice acceptor (adenovirus)UNSA
  • achondrogenesis type II(MedGEN)

  • avascular necrosis of femoral head, primary, 1(MedGEN)

  • Czech dysplasia, metatarsal type(MedGEN)
    • more 13 Diseases
  • Kniest dysplasia(DisGeNET, MedGEN)

  • Legg-Calve-Perthes disease(MedGEN)

  • mild spondyloepiphyseal dysplasia due to COL2A1 mutation with early-onset osteoarthritis(MedGEN)

  • multiple epiphyseal dysplasia, Beighton type(MedGEN)

  • platyspondylic dysplasia, Torrance type(MedGEN)

  • spondyloepimetaphyseal dysplasia, Strudwick type(MedGEN)

  • spondyloepiphyseal dysplasia congenita(MedGEN)

  • spondyloepiphyseal dysplasia, Stanescu type(MedGEN)

  • spondylometaphyseal dysplasia, 'corner fracture' type(MedGEN)

  • spondyloperipheral dysplasia(MedGEN)

  • Stickler syndrome type 1(MedGEN)

  • Stickler syndrome, type I, nonsyndromic ocular(MedGEN)

  • vitreoretinopathy with phalangeal epiphyseal dysplasia(MedGEN)
    • lacZbeta-galactosidase (E. coli)UN
  • achondrogenesis type II(MedGEN)

  • avascular necrosis of femoral head, primary, 1(MedGEN)

  • Czech dysplasia, metatarsal type(MedGEN)
    • more 13 Diseases
  • Kniest dysplasia(DisGeNET, MedGEN)

  • Legg-Calve-Perthes disease(MedGEN)

  • mild spondyloepiphyseal dysplasia due to COL2A1 mutation with early-onset osteoarthritis(MedGEN)

  • multiple epiphyseal dysplasia, Beighton type(MedGEN)

  • platyspondylic dysplasia, Torrance type(MedGEN)

  • spondyloepimetaphyseal dysplasia, Strudwick type(MedGEN)

  • spondyloepiphyseal dysplasia congenita(MedGEN)

  • spondyloepiphyseal dysplasia, Stanescu type(MedGEN)

  • spondylometaphyseal dysplasia, 'corner fracture' type(MedGEN)

  • spondyloperipheral dysplasia(MedGEN)

  • Stickler syndrome type 1(MedGEN)

  • Stickler syndrome, type I, nonsyndromic ocular(MedGEN)

  • vitreoretinopathy with phalangeal epiphyseal dysplasia(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal articular cartilage morphology(MP:0006433)

  • decreased susceptibility to induced arthritis(MP:0003436)

  • increased chondrocyte proliferation(MP:0014100)

  • increased collagen level(MP:0020253)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Col2a1 promoter-enhancer, adenovirus splice acceptor (SA), mouse Ctgf cDNA with HA tag cDNA, human influenza virus HA (hemagglutinin) tag sequence, EMCV Internal ribosomal enrty site (ires), E.coli lacZ, SV40 polyA signal
    Research applicationCancer Research
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2013 8(3):e59226.　　PLoS One. 2013 8(8):e71156.For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITORS and must obtain a prior written consent from the DEPOSITORS.　　The RECIPIENT must contact the DEPOSITORS in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasaharu Takigawa (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data