RBRC06327

Information1

Image
BRC No.RBRC06327
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Ffar3<tm1Reh>
Former Common nameGpr41 KO, B6-Ffar3 KO
H-2 Haplotype
ES Cell lineSM1[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells derived from 129S6/SvEvTac mice were used to generate the mutant mice. The mice were crossed to C57BL/6.
Strain descriptionThe Gpr41 (Ffar3) exon coding region was replaced with a nLacZ and floxed neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crjl)]
ReferencesProc. Natl. Acad. Sci. USA, 105, 16767-1672 (2008). 18931303

Health Report

Examination Date / Room / Rack2015/04/06Room:3-3Rack:A
2015/02/02Room:3-3Rack:A
2014/12/01Room:3-3Rack:A
2014/10/06Room:3-3Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ffar3free fatty acid receptor 37Ffar3<tm1Reh>targeted mutation 1, Robert E Hammer

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)7nls

Information2

Donor DNASV40 large T antigen nuclear localization signal (NLS), E. coli lacZ, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Ffar3 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A. 105, 16767-1672 (2008).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data