Strain Data Sheet

RBRC06325

Strain Information

Image
BRC No.RBRC06325
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nfatc2<tm1Rao> Tob1<tm1Tya>
Former Common nameNFAT-c2/Tob-1 double KO, DKO, B6-Nfatc2/Tob1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Jamie Modiano, University of Minnesota. The double mutant mice were generated by crossing with Nfatc2 KO (Anjana Rao, Harvard Medical School) and Tob1 KO(Tadashi Yamamoto, The Institute of Medical Science, Tokyo University).
Strain descriptionNfatc2 and Tob1 double knockout mice. Approximately 50% of Nfatc2 knockout mice develop B-cell lymphomas by 15-18 months of age. But, Nfatc2 and Tob1 double knockout mice do not have obvious gross phenotypes up to 6 months of age. Salivary glands showed lymphoid infiltration into the parenchyma, but no more severe than that observed in single Nfatc2 knockout mice, suggesting Tob1 neither accelerates nor delays lymphoid hyper-reactivity observed under conditions of Nfatc2 deficiency. Nfatc2 homozygous and Tob1 heterozygous dams generally fail to produce milk (so pups must be fostered). Histologically, adult female Nfatc2 homozygous/Tob1 heterozygous mice show comparable mammary development as wild type B6 mice, suggesting the inability to nurse pups is not due to anatomic defects in mammary development. Matings using Tob1 homozygous males created larger pups and more dystocias in the females.
Colony maintenanceNfatc2: Homozygote x Homozygote; Tob1: Wild-type (female) x Heterozygote (male) [or Crossing to C57BL/6J(Crlj)]
References
Negative regulation of BMP/Smad signaling by Tob in osteoblasts.
Yoshida Y, Tanaka S, Umemori H, Minowa O, Usui M, Ikematsu N, Hosoda E, Imamura T, Kuno J, Yamashita T, Miyazono K, Noda M, Noda T, Yamamoto T
Cell, 103, 1085-1097 (2000). 11163184

Nfatc2 and Tob1 have non-overlapping function in T cell negative regulation and tumorigenesis.
May S L, Zhou Q, Lewellen M, Carter C M, Coffey D, Highfill S L, Bucher C M, Matise I, Morse H C 3rd, O'Sullivan M G, Schutten M, Johnson C, Bellgrau D, Blazar B R, Modiano J F
PLoS One, 9, e100629 (2014). 24945807

An enhanced immune response in mice lacking the transcription factor NFAT1.
Xanthoudakis S, Viola J P, Shaw K T, Luo C, Wallace J D, Bozza P T, Luk D C, Curran T, Rao A
Science, 272, 892-895 (1996). 8629027

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tob1transducer of ErbB-2.111Tob1targeted mutation 1, Tadashi Yamamoto
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)
Nfatc2nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 22Nfatc2targeted mutation 1, Anjana Rao
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bone ossification(MP:0008271)

  • abnormal mast cell physiology(MP:0002423)

  • abnormal osteoblast differentiation(MP:0008395)

  • decreased interleukin-13 secretion(MP:0008673)

  • decreased interleukin-4 secretion(MP:0008700)
    • more 5 phenotypes
  • decreased tumor necrosis factor secretion(MP:0008561)

  • increased bone mass(MP:0005605)

  • increased cell proliferation(MP:0000351)

  • increased osteoblast cell number(MP:0004988)

  • increased trabecular bone mass(MP:0010872)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Nfatc2 genomic DNA, mouse Tob1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene
    Research application
    Specific Term and Conditions1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLOS One, 9, e100629 (2014). 2) Recipient will not backcross the BIOLOGICAL RESOURCE to establish breeding colonies for either the NFAT1 KO mice or the Tob-1KO mice. 3) Tob-1 KO mice are the property of the Okinawa Institute of Science and Technology Graduate University and are available from the RIKEN BRC. The RECIPIENT must inform the DEVELOPER (Professor Tadashi Yamamoto, Okinawa Institute of Science and Technology Graduate University) the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from him. The RECIPIENT agrees to use the BIOLOGICAL RESOUCE as collaboration with the DEVELOPER in case his ongoing research is overlapping with that of the RECIPIENT. 4) NFAT1 KO mice are the property of the LaJolla Institute for Allergy and Immunology and will not be further distributed to others. Any requests for the NFAT1 KO mice shall be referred to the LaJolla Institute for Allergy and Immunology. 5) Recipient shall acquire prior written consent from the Depositor for use of the BIOLOGICAL RESOURCE by for-profit institutions or for commercial research purposes. 6) Recipient shall acquire prior written consent from the Depositor before filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    DepositorJaime F. Modiano (University of Minnesota)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data