Strain Data Sheet

RBRC06322

Strain Information

Image
BRC No.RBRC06322
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Edn1<tm1Ywa>
Former Common nameET-1 flox (ET1 flox), B6-Edn1 floxed, Endothelin 1(ET-1) conditional knockout without transgene (or Flox ET-1) mouse
H-2 Haplotype
ES Cell lineSM1[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells were used to generate the mutant mice.
Strain descriptionET1 (Edn1 / endothelin 1) gene floxed mice. Exon 2 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
References
Low blood pressure in endothelial cell-specific endothelin 1 knockout mice.
Kisanuki Y Y, Emoto N, Ohuchi T, Widyantoro B, Yagi K, Nakayama K, Kedzierski R M, Hammer R E, Yanagisawa H, Williams S C, Richardson J A, Suzuki T, Yanagisawa M
Hypertension 56, 121-128 (2010). 20516397

Disruption of ECE-1 and ECE-2 reveals a role for endothelin-converting enzyme-2 in murine cardiac development.
Yanagisawa H, Hammer R E, Richardson J A, Emoto N, Williams S C, Takeda S i, Clouthier D E, Yanagisawa M
J. Clin. Invest., 114, 4, 504-511 (2004). 10811845

Mice with cardiomyocyte-specific disruption of the endothelin-1 gene are resistant to hyperthyroid cardiac hypertrophy.
Shohet R V, Kisanuki Y Y, Zhao X S, Siddiquee Z, Franco F, Yanagisawa M
Proc. Natl. Acad. Sci. USA, 101, 2088-2093 (2004). 14764893

Health Report

Examination Date / Room / Rack2023/02/06Room:2-検Rack:C
2022/11/07Room:2-検Rack:C

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Edn1endothelin 113Edn1targeted mutation 1, Masashi Yanagisawa
  • auriculocondylar syndrome 3(MedGEN)

  • question mark ears, isolated(MedGEN)
    • loxPphage P1 loxP13loxP
  • auriculocondylar syndrome 3(MedGEN)

  • question mark ears, isolated(MedGEN)
    • loxPphage P1 loxP13loxP
  • auriculocondylar syndrome 3(MedGEN)

  • question mark ears, isolated(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Edn1 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Hematological Research
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A., 101, 2088-2093 (2004).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data