Strain Data Sheet

RBRC06321

Strain Information

Image
BRC No.RBRC06321
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ece2<tm1Ywa>
Former Common nameECE-2/C57, B6-Ece2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells were used to generate the mutant mice. The mutant mice were crossed to C57BL/6.
Strain descriptionECE-2 (Ece1 / endothelin converting enzyme 2) gene knockout mice. Exon 14 and 15 were replaced with a neomycin resistant cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Disruption of ECE-1 and ECE-2 reveals a role for endothelin-converting enzyme-2 in murine cardiac development.
Yanagisawa H, Hammer R E, Richardson J A, Emoto N, Williams S C, Takeda S i, Clouthier D E, Yanagisawa M
J. Clin Invest., 105, 1373-1382 (2000). 10811845

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ece2endothelin converting enzyme 216Ece2targeted mutation 1, Masashi Yanagisawa
GHGrowth hormone polyA (Bovine)16GH
neoneomycin resistance gene (E. coli)16mouse RNA polymerase II promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, mouse RNA polymerase II promoter, bovine growth hormone polyadenylation site, mouse Ece2 genomic DNA
    Research applicationDevelopmental Biology Research
    Hematological Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin Invest., 105, 1373-1382 (2000).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data