RBRC06318

Information1

Image
BRC No.RBRC06318
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameBALB/c-Cables1<tm1Utr>
Former Common nameBALB-Cables1 KO
H-2 Haplotype
ES Cell line
Background strainBALB/cAnNCrlCrlj
Appearance
Strain developmentDeveloped by Fumihiro Sugiyama, Laboratory animal resource center, University of Tsukuba in 2014. BALB/c derived ES cells were used to generate the knockout mice.
Strain descriptionCables1 gene null knockout mice. Exon 1 was replaced with a neo cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAnNCrlCrlj]
ReferencesLaboratory Investigation, 94, 321-330 (2014) . 24336072

Health Report

Examination Date / Room / Rack2015/03/23Room:4-BRack:A
2015/01/19Room:4-BRack:A
2014/11/17Room:4-BRack:A
2014/09/16Room:4-BRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cables1CDK5 and Abl enzyme substrate 118Cables1<tm1Utr>targeted mutation 1, Ken-ichi Yagami

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18human phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Cables1 genomic DNA, human phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, phage P1 loxP, yeast FRT sites
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Truncated Cables1 Causes agenesis of the corpus callosum in mice.
DepositorFumihiro Sugiyama (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data