Strain Data Sheet

RBRC06317

Strain Information

Image
BRC No.RBRC06317
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameC.Cg-Cables1<Tg(CAG-rtTA,CAG-tTS)138Utr>
Former Common nameTAS, BALB-Cables1/TAS
H-2 Haplotype
ES Cell line
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Fumihiro Sugiyama, Laboratory animal resource center, University of Tsukuba in 2010. The transgene was coinjected into the pronuclei of B6D2F1 fertilized eggs. The transgenic mice were backcrossed to BALB/cAJcl for 14 times.
Strain descriptionInsertional mutant mice of the Cables1 gene, arose from CAG-rtTA/CAG-tTS transgenic lines. Tg homozygous mice and about 50% of hemizygous mice show agenesis of the corpus callosum (ACC).
Colony maintenanceCarrier x Noncarrier [BALB/cAJcl]
References
A novel locus on proximal chromosome 18 associated with agenesis of the corpus callosum in mice.
Mizuno S, Mizobuchi A, Iseki H, Iijima S, Matsuda Y, Kunita S, Sugiyama F, Yagami K
Mammalian Genome, 21, 525-533 (2010) . 20967545

Truncated Cables1 causes agenesis of the corpus callosum in mice.
Mizuno S, Tra D T, Mizobuchi A, Iseki H, Mizuno-Iijima S, Kim J D, Ishida J, Matsuda Y, Kunita S, Fukamizu A, Sugiyama F, Yagami K
Laboratory Investigation, 94, 321-330 (2014) . 24336072

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cables1CDK5 and Abl enzyme substrate 118Cables1transgene insertion 1, University of Tsukuba Laboratory Animal Resource Center
rtTAE. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)18rtTACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
tTSE. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA18tTSCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal CNS glial cell morphology(MP:0000952)

  • abnormal forebrain morphology(MP:0000783)

  • absent corpus callosum(MP:0002196)

  • circling(MP:0001394)

  • decreased anterior commissure size(MP:0008226)
    • more 2 phenotypes
  • decreased hippocampal commissure size(MP:0008222)

  • enlarged lateral ventricles(MP:0008535)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), rtTA[E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain], tTS (E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA)
    Research applicationDevelopmental Biology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Truncated Cables1 Causes agenesis of the corpus callosum in mice.
    DepositorFumihiro Sugiyama (University of Tsukuba)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data