RBRC06315

Information1

Image
BRC No.RBRC06315
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameMF1.129-Sparc<tm1Cam>
Former Common nameMF1-Sparc KO
H-2 Haplotype
ES Cell lineCCB [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by William H. Colledge, University of Cambridge. CCB ES cells were used. Maintained by Yasumasa Kato, Ohu university School of Dentistry. C3H congenic (over N9 generation) (RBRC06314), MF1 mixed background (RBRC06315).
Strain descriptionSparc (osteonectin, BM-40) gene knockout mice. Exon 6 of Sparc gene was replaced with a SA-lacZ/PGK-neo cassette. Homozygous mutant mice exhibit cataracts.
Colony maintenanceHomozygote x Homozygote [or Crossing to HsdOla:MF1]
ReferencesEMBO J., 17, 1860-1870 (1998). 9524110
Int. J. Oncol., 27, 759-768 (2005). 16077926

Health Report

Examination Date / Room / Rack2018/10/01Room:3-5Rack:B
2018/08/06Room:3-5Rack:B
2018/06/04Room:3-5Rack:B
2018/04/02Room:3-5Rack:B
2018/02/05Room:3-5Rack:B
2017/12/04Room:3-5Rack:B
2017/10/02Room:3-5Rack:B
2017/08/21Room:3-5Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sparcsecreted acidic cysteine rich glycoprotein11Sparc<tm1Cam>targeted mutation 1, University of Cambridge

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor (adenovirus)11SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Sparc genomic DNA, adenovirus splice acceptor, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycine resistance gene, bovine growth hormone gene polyadenylation signal
Research applicationCancer Research
Cell Biology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Hematological Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 17, 1860-1870 (1998).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYasumasa Kato (Kanagawa Dental College)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data