Strain Data Sheet

RBRC06315

Strain Information

Image
BRC No.RBRC06315
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameMF1.129-Sparc<tm1Cam>
Former Common nameMF1-Sparc KO
H-2 Haplotype
ES Cell lineCCB [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by William H. Colledge, University of Cambridge. CCB ES cells were used. Maintained by Yasumasa Kato, Ohu university School of Dentistry. C3H congenic (over N9 generation) (RBRC06314), MF1 mixed background (RBRC06315).
Strain descriptionSparc (osteonectin, BM-40) gene knockout mice. Exon 6 of Sparc gene was replaced with a SA-lacZ/PGK-neo cassette. Homozygous mutant mice exhibit cataracts.
Colony maintenanceHomozygote x Homozygote [or Crossing to HsdOla:MF1]
References
Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens.
Gilmour D T, Lyon G J, Carlton M B, Sanes J R, Cunningham J M, Anderson J R, Hogan B L, Evans M J, Colledge W H
EMBO J., 17, 1860-1870 (1998). 9524110

Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation after genetic disruption of secreted protein acidic and rich in cysteine.
Kato Y, Tsukuda M, Nagashima Y, Koshika S, Sakai N, Yao M, Kubota Y, Aoki I, Colledge W H, Foidart J M, Hata R, Thompson E W
Int. J. Oncol., 27, 759-768 (2005). 16077926

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GHGrowth hormone polyA (Bovine)11GH
  • osteogenesis imperfecta type 17(MedGEN)
    • SASplice acceptor (adenovirus)11SA
  • osteogenesis imperfecta type 17(MedGEN)
    • Sparcsecreted acidic cysteine rich glycoprotein11Sparctargeted mutation 1, University of Cambridge
  • osteogenesis imperfecta type 17(MedGEN)
    • lacZbeta-galactosidase (E. coli)11
  • osteogenesis imperfecta type 17(MedGEN)
    • neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)
  • osteogenesis imperfecta type 17(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal retina layer morphology(MP:0003727)

  • subcapsular cataract(MP:0010251)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Sparc genomic DNA, adenovirus splice acceptor, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycine resistance gene, bovine growth hormone gene polyadenylation signal
    Research applicationCancer Research
    Cell Biology Research
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Hematological Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 17, 1860-1870 (1998).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYasumasa Kato (Kanagawa Dental College)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data