RBRC06313

Information1

Image
BRC No.RBRC06313
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Sparc<tm1Hwe>
Former Common nameB6-Sparc KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Chin Chen Howem The Wistar Institute. CCB ES cells were used to generate the knockout mice. The mutant mice were maintained by Yasumasa Kato, Ohu university School of Dentistry through Helene Sga, Benaroya Research Institute at Virginia. C57BL/6 congenic.
Strain descriptionSparc (osteonectin, BM-40) gene knockout mice. Exon 4 of Sparc gene was replaced with MC1-neo and Pol2-neo tandem cassettes. Homozygous mutant mice exhibit cataracts, abnormal skeletal morphology and osteopenia (viable and fertile).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesExp. Eye Res. 68, 321-331 (1999). 10079140
Invest Ophthalmol Vis Sci. 39, 2674-2680 (1998). 9856777

Health Report

Examination Date / Room / Rack2014/09/16Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sparcsecreted acidic cysteine rich glycoprotein11Sparc<tm1Hwe>targeted mutation 1, Chin Chen Howe

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse RNA polymerase II promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNApolyoma virus thymidine kinase gene promoter, E. coli neomycine resistance gene, mouse RNA polymerase II promoter, bovine growth hormone gene polyadenylation signal, mouse Sparc genomic DNA
Research applicationCancer Research
Cell Biology Research
Developmental Biology Research
Hematological Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Invest. Ophthalmol. Vis. Sci., 39, 2674-2680 (1998 ).
DepositorYasumasa Kato (Kanagawa Dental College)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data