Strain Data Sheet

RBRC06313

Strain Information

Image
BRC No.RBRC06313
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Sparc<tm1Hwe>
Former Common nameB6-Sparc KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Chin Chen Howem The Wistar Institute. CCB ES cells were used to generate the knockout mice. The mutant mice were maintained by Yasumasa Kato, Ohu university School of Dentistry through Helene Sga, Benaroya Research Institute at Virginia. C57BL/6 congenic.
Strain descriptionSparc (osteonectin, BM-40) gene knockout mice. Exon 4 of Sparc gene was replaced with MC1-neo and Pol2-neo tandem cassettes. Homozygous mutant mice exhibit cataracts, abnormal skeletal morphology and osteopenia (viable and fertile).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Disruption of the Sparc locus in mice alters the differentiation of lenticular epithelial cells and leads to cataract formation.
Bassuk J A, Birkebak T, Rothmier J D, Clark J M, Bradshaw A, Muchowski P J, Howe C C, Clark J I, Sage E H
Exp. Eye Res. 68, 321-331 (1999). 10079140

SPARC deficiency leads to early-onset cataractogenesis.
Norose K, Clark J I, Syed N A, Basu A, Heber-Katz E, Sage E H, Howe C C
Invest. Ophthalmol. Vis. Sci., 39, 2674-2680 (1998). 9856777

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GHGrowth hormone polyA (Bovine)11GH
  • osteogenesis imperfecta type 17(MedGEN)
    • Sparcsecreted acidic cysteine rich glycoprotein11Sparctargeted mutation 1, Chin Chen Howe
  • osteogenesis imperfecta type 17(MedGEN)
    • neoneomycin resistance gene (E. coli)11mouse RNA polymerase II promoter
  • osteogenesis imperfecta type 17(MedGEN)
    • neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • osteogenesis imperfecta type 17(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal bone remodeling(MP:0002998)

  • abnormal bone strength(MP:0001542)

  • abnormal lens capsule morphology(MP:0003236)

  • abnormal lens epithelium morphology(MP:0003237)

  • abnormal lens fiber morphology(MP:0002840)
    • more 20 phenotypes
  • abnormal lens morphology(MP:0001303)

  • abnormal osteoblast morphology(MP:0004986)

  • abnormal osteoclast morphology(MP:0004982)

  • abnormal skeleton morphology(MP:0005508)

  • abnormal skeleton physiology(MP:0001533)

  • abnormal trabecular bone morphology(MP:0000130)

  • cortical cataract(MP:0010255)

  • decreased bone mineral density(MP:0000063)

  • decreased locomotor activity(MP:0001402)

  • decreased osteoblast cell number(MP:0004989)

  • decreased osteoclast cell number(MP:0004985)

  • delayed wound healing(MP:0002908)

  • enhanced wound healing(MP:0002724)

  • eye inflammation(MP:0001851)

  • increased tumor growth/size(MP:0003721)

  • posterior subcapsular cataract(MP:0010253)

  • retina degeneration(MP:0001326)

  • retina detachment(MP:0003099)

  • ruptured lens capsule(MP:0008841)

  • vitreous body inflammation(MP:0006201)
    • Detailed phenotype data

    Ordering Information

    Donor DNApolyoma virus thymidine kinase gene promoter, E. coli neomycine resistance gene, mouse RNA polymerase II promoter, bovine growth hormone gene polyadenylation signal, mouse Sparc genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Developmental Biology Research
    Hematological Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Invest. Ophthalmol. Vis. Sci., 39, 2674-2680 (1998).
    DepositorYasumasa Kato (Kanagawa Dental College)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data