RBRC06307

Information1

Image
BRC No.RBRC06307
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129(B6)-Mafk<tm1.1Jeng>
Former Common nameMafK KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by University of Michigan, Douglas Engel and Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba. R1 RS cells were used for generation of the mice. 129, C57BL/6 and ICR mixed background. A MC1-TK MC1-neo cassette of MafF KO (RBRC06305) and MafG KO (RBRC06306) mice was removed by crossing with Ayu1-cre mice. A PGK-neo cassette of MafK KO mice (RBRC06307) was removed by crossing with Ayu1-cre mice.
Strain descriptionSmall Maf protein genes knockout mice (MafF: RBRC06305, MafG: RBRC06306 and MafK: RBRC06307). Both MafF and MafK homozygous mutant mice show no obvious abnormality. MafG homozygous mutant mice exhibit impaired platelet formation accompanied by megakaryocyte proliferation, as well as mild behavioral abnormalities. MafF, MafG and MafK triple homozygous mutant mice are embryonic lethal (around E11.5).
Colony maintenanceHomozygote x Homozygote [or Crossing to Jcl:ICR]
ReferencesGenes Dev., 12, 2164-2174 (1998). 9679061

Health Report

Examination Date / Room / Rack2017/04/17Room:3-DRack:C
2017/02/20Room:3-DRack:C
2016/12/19Room:3-DRack:C
2016/10/17Room:3-DRack:C
2016/08/22Room:3-DRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mafkv-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian)5Mafk<tm1.1Jeng>targeted mutation 1.1, James Douglas Engel

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)5nls

Information2

Donor DNASV40 large T antigen nuclear localization signal (NLS), E. coli lacZ, phage P1 loxP site, mouse Mafk genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 12, 2164-2174 (1998).
DepositorMasayuki Yamamoto (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data