Strain Data Sheet

RBRC06307

Strain Information

Image
BRC No.RBRC06307
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129(B6)-Mafk<tm1.1Jeng>
Former Common nameMafK KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by University of Michigan, Douglas Engel and Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba. R1 RS cells were used for generation of the mice. 129, C57BL/6 and ICR mixed background. A MC1-TK MC1-neo cassette of MafF KO (RBRC06305) and MafG KO (RBRC06306) mice was removed by crossing with Ayu1-cre mice. A PGK-neo cassette of MafK KO mice (RBRC06307) was removed by crossing with Ayu1-cre mice.
Strain descriptionSmall Maf protein genes knockout mice (MafF: RBRC06305, MafG: RBRC06306 and MafK: RBRC06307). Both MafF and MafK homozygous mutant mice show no obvious abnormality. MafG homozygous mutant mice exhibit impaired platelet formation accompanied by megakaryocyte proliferation, as well as mild behavioral abnormalities. MafF, MafG and MafK triple homozygous mutant mice are embryonic lethal (around E11.5).
Colony maintenanceHomozygote x Homozygote [or Crossing to Jcl:ICR]
References
Impaired megakaryopoiesis and behavioral defects in mafG-null mutant mice.
Shavit J A, Motohashi H, Onodera K, Akasaka J, Yamamoto M, Engel J D
Genes Dev., 12, 2164-2174 (1998). 9679061

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mafkv-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian)5Mafktargeted mutation 1.1, James Douglas Engel
lacZbeta-galactosidase (E. coli)5
loxPphage P1 loxP5loxP
nlsSV40 large T antigen nuclear localization signal (NLS)5nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNASV40 large T antigen nuclear localization signal (NLS), E. coli lacZ, phage P1 loxP site, mouse Mafk genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 12, 2164-2174 (1998).
    DepositorMasayuki Yamamoto (Tohoku University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data