RBRC06302

Information1

Image
BRC No.RBRC06302
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameNEAT1(lacZ)(Acc.No.CDB0773K)
Former Common nameNEAT1 KO, B6-Neat1 KO, NEAT1(lacZ)(Acc.No.CDB0773K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Nakagawa, RIKEN Advanced Science Institute. TT2 ES cells were used to generate the knockout mice. A FRT-flanked neo cassette was removed by crossing with FLP mice. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionNeat1 ncRNA knock-out mice. A lacZ was inserted immediately downstream of the transcription start site of Neat1.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesJ. Cell Biol., 193, 31-39 (2011). 21444682

Health Report

Examination Date / Room / Rack2014/09/08Room:2-検Rack:N
2014/07/07Room:2-検Rack:N

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Neat1nuclear paraspeckle assembly transcript 1 (non-protein coding)19Neat1<tm1.1Shna>targeted mutation 1.1, Shinichi Nakagawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site19Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)19

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence19lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Information2

Donor DNAPhage P1 lox71 site, E. coli lacZ, rabbit beta globin polyA, yeast FRT (flipase recombination target) site, Phage P1 loxP site, PGK1 polyA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Shinichi Nakagawa (nakagawas@riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No.CDB0773K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (will be announced). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data