Strain Data Sheet
RBRC06301
Strain Information | |
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| Image | |
| BRC No. | RBRC06301 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Batf<tm1(GFP)> |
| Former Common name | B6 BATF KO, BATF-GFP knock-in, C57BL/6-Batf<tm1(GFP)> |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | C57BL/6NCrlCrlj |
| Appearance | |
| Strain development | Developed by Yoshiko Iwai, Tokyo Medical and Dental University (University of Occupational and Environmental Health, Japan). RENKA ES cells were used to generate the knockout mice. C57BL/6 background. |
| Strain description | Batf-GFP knockin-mice. The coding sequences of Batf gene were replaced by the GFP. Homozygous mutant mice show immunodeficiency. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] |
| References | Basic leucine zipper transcription factor, ATF-like (BATF) regulates epigenetically and energetically effector CD8 T-cell differentiation via Sirt1 expression. Kuroda S, Yamazaki M, Abe M, Sakimura K, Takayanagi H, Iwai Y Proc. Natl. Acad. Sci. USA, 108, 14885-14889 (2011). 21873234 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| SV40 polyA signal | 12 | ||||||
| Batf | basic leucine zipper transcription factor, ATF-like | 12 | Batf | targeted mutation 1, Yoshiko Iwai | |||
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 12 | EGFP | ||||
| neo | neomycin resistance gene (E. coli) | 12 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 1 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Jellyfish Enhanced Green Fluorescent Protein(EGFP)gene cDNA, SV40 PolyA signal, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase PolyA signal, mouse Batf genomic DNA |
| Research application | Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
| Specific Term and Conditions | 1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2011 108(36):14885-9. 3) A material transfer agreement specified by DEPOSITOR and RIKEN BRC will be used for the transfer of the BIOLOGICAL RESOURCE from RIKEN BRC to the RECIPIENT. 4) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 5) Upon completion or discontinuation of the research specified in the MTA, the RECIPIENT shall promptly report to the DEPOSITOR any results from its research using the BIOLOGICAL RESOURCE (hereinafter "RESEARCH RESULT"). 6) The RECIPIENT agrees that the DEPOSITOR shall be free to use the RESEARCH RESULT for research purpose without any compensation. 7) The RECIPIENT shall not distribute, sell or assign the BIOLOGICAL RESOURCE or any part thereof, including without limitation those contained in modification or cross-bred mouse, to any third party. 8) RECIPIENT shall notify the DEPOSITOR upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. |
| Depositor | Yoshiko Iwai (University of Occupational and Environmental Health) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |