RBRC06301

Information1

Image
BRC No.RBRC06301
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Batf<tm1(GFP)>
Former Common nameB6 BATF KO, BATF-GFP knock-in, C57BL/6-Batf<tm1(GFP)>
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Yoshiko Iwai, Tokyo Medical and Dental University (University of Occupational and Environmental Health, Japan). RENKA ES cells were used to generate the knockout mice. C57BL/6 background.
Strain descriptionBatf-GFP knockin-mice. The coding sequences of Batf gene were replaced by the GFP. Homozygous mutant mice show immunodeficiency.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesProc. Natl. Acad. Sci. USA, 108, 14885-14889 (2011). 21873234

Health Report

Examination Date / Room / Rack2014/09/08Room:2-検Rack:G
2014/07/07Room:2-検Rack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Batfbasic leucine zipper transcription factor, ATF-like12Batf<tm1(GFP)Yiwai>targeted mutation 1, Yoshiko Iwai

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)12EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal12

Information2

Donor DNAJellyfish Enhanced Green Fluorescent Protein(EGFP)gene cDNA, SV40 PolyA signal, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase PolyA signal, mouse Batf genomic DNA
Research applicationFluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2011 108(36):14885-9. 3) A material transfer agreement specified by DEPOSITOR and RIKEN BRC will be used for the transfer of the BIOLOGICAL RESOURCE from RIKEN BRC to the RECIPIENT. 4) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 5) Upon completion or discontinuation of the research specified in the MTA, the RECIPIENT shall promptly report to the DEPOSITOR any results from its research using the BIOLOGICAL RESOURCE (hereinafter "RESEARCH RESULT"). 6) The RECIPIENT agrees that the DEPOSITOR shall be free to use the RESEARCH RESULT for research purpose without any compensation. 7) The RECIPIENT shall not distribute, sell or assign the BIOLOGICAL RESOURCE or any part thereof, including without limitation those contained in modification or cross-bred mouse, to any third party. 8) RECIPIENT shall notify the DEPOSITOR upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorYoshiko Iwai (University of Occupational and Environmental Health)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data