RBRC06297

Information1

Image
BRC No.RBRC06297
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameCAJcl.Cg-Aicda<tm1Hon>
Former Common nameBALBcAJcl-AID KO, BALBcA-AID KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Reiko Shinkura, Nagahama Institute of Bio-Science and Technology, and Tasuku Honjo, Graduate School of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to BALB/cAnPt over 5 times, and then nackcrossed to BALB/cAJcl.
Strain descriptionAicda knockout mice. BALB/cAJcl background. Exon 2 and part of Exon 3 were replaced with a neomycin resistant cassette. For the details, please contact the developer (Dr. Reiko Shinkura, Nagahama Institute of Bio-Science and Technology).
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAJcl]
ReferencesScience, 298, 1424-1427 (2002). 12434060
Cell, 102, 553-563 (2000). 11007474

Health Report

Examination Date / Room / Rack2015/02/16Room:3-ARack:G
2014/12/15Room:3-ARack:G
2014/10/20Room:3-ARack:G
2014/08/18Room:3-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Aicdaactivation-induced cytidine deaminase6Aicda<tm1Hon>targeted mutation 1, Tasuku Honjo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, E. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter (PGK promoter), mouse Aicda genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science, 298, 1424-1427 (2002).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Honjo Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data