Strain Data Sheet


Strain Information

BRC No.RBRC06294
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Aqp7<tm1Tfun>
Former Common nameAqp7 KO, B6-Aqp7 KO
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Norikazu Maeda, Tohru Funahashi and Iichiro Shimomura. 129 derived ES cells were used. The mutant mice were crossed to C57BL/6 over 5 times.
Strain descriptionAquaporin 7 (Aqp7) gene knockout mice. Exon 1-3 containing the translation start site was replaced with a neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesProc. Natl. Acad. Sci. USA, 102, 10993-10998 (2005). 16009937
Proc. Natl. Acad. Sci. USA, 101, 17801-17806 (2004). 15591341
Cardiovasc. Res., 83, 34-41 (2009). 19297367

Health Report

Examination Date / Room / Rack2023/07/24Room:3-CRack:G


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aqp7aquaporin 74Aqp7<tm1Tfun>targeted mutation 1, Tohru Funahashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk1 polyA, mouse Aqp7 genomic DNA
Research applicationCardiovascular Research
Cre/loxP system
Metabolism Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Nat.l Acad. Sci. USA, 101, 17801-17806 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorIichiro Shimomura (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Ishihama S, Yoshida S, Yoshida T, Mori Y, Ouchi N, Eguchi S, Sakaguchi T, Tsuda T, Kato K, Shimizu Y, Ohashi K, Okumura T, Bando YK, Yagyu H, Wettschureck N, Kubota N, Offermanns S, Kadowaki T, Murohara T, Takefuji M.
LPL/AQP7/GPD2 promotes glycerol metabolism under hypoxia and prevents cardiac dysfunction during ischemia.
FASEB J 35(12) e22048(2021) 34807469

Fujii M, Bessho R, Akimoto T, Ishii Y.
Cardioprotective effect of St. Thomas' Hospital No. 2 solution against age-related changes in aquaporin-7-deficient mice.
Gen Thorac Cardiovasc Surg (2023) 37691043

Fujii M, Ota K, Bessho R.
Cardioprotective effect of hyperkalemic cardioplegia in an aquaporin 7-deficient murine heart.
Gen Thorac Cardiovasc Surg 68(6) 578-584(2020) 31707553

Raje V, Ahern KW, Martinez BA, Howell NL, Oenarto V, Granade ME, Kim JW, Tundup S, Bottermann K, Gödecke A, Keller SR, Kadl A, Bland ML, Harris TE.
Adipocyte lipolysis drives acute stress-induced insulin resistance.
Sci Rep 10(1) 18166(2020) 33097799