Strain Data Sheet

RBRC06294

Information1

Image
BRC No.RBRC06294
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Aqp7<tm1Tfun>
Former Common nameAqp7 KO, B6-Aqp7 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Norikazu Maeda, Tohru Funahashi and Iichiro Shimomura. 129 derived ES cells were used. The mutant mice were crossed to C57BL/6 over 5 times.
Strain descriptionAquaporin 7 (Aqp7) gene knockout mice. Exon 1-3 containing the translation start site was replaced with a neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesProc. Natl. Acad. Sci. USA, 101, 17801-17806 (2004). 15591341
Cardiovasc. Res., 83, 34-41 (2009). 19297367
Proc. Natl. Acad. Sci. USA, 102, 10993-10998 (2005). 16009937

Health Report

Examination Date / Room / Rack2022/07/25Room:3-CRack:G
2022/04/25Room:3-CRack:G
2022/02/14Room:3-CRack:G
2021/11/15Room:3-CRack:G
2021/08/16Room:3-CRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aqp7aquaporin 74Aqp7<tm1Tfun>targeted mutation 1, Tohru Funahashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk1 polyA, mouse Aqp7 genomic DNA
Research applicationCardiovascular Research
Cre/loxP system
covid19_progression
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Nat.l Acad. Sci. USA, 101, 17801-17806 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorIichiro Shimomura (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data