RBRC06293

Information1

Image
BRC No.RBRC06293
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ngly1<tm1.1Tasuz>
Former Common namePNGase KO, B6-Ngly1 KO
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Tadashi Suzuki, Global Research Cluster, RIKEN. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Exons 11 and 12 of Ngly gene were removed by crossing with Cre mice (B6). The mutant mice were backcrosses to C57BL/6 over 13 times.
Strain descriptionNgly gene knockout mice. Exons 11 and 12 were removed. Homozygous mutant mice are lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crj)]
ReferencesPLoS Genet. 3(4):e1006696 (2017). 28426790

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ngly1N-glycanase 14Ngly1<tm1.1Tasuz>targeted mutation 1.1, Tadashi Suzuki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP4loxP

Information2

Donor DNAPhage P1 loxP site, yeast FRT (flipase recombination target) site, mouse Ngly1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet. 2017 Apr 20;13(4):e1006696. doi: 10.1371/journal.pgen.1006696. eCollection 2017 Apr.
DepositorTadashi Suzuki (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data