RBRC06291

Information1

Image
BRC No.RBRC06291
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ube3c<tm1b(EUCOMM)Hmgu>/B01
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ube3cubiquitin protein ligase E3C5Ube3c<tm1b(EUCOMM)Hmgu>targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal5

Information2

Donor DNAyeast FRT (flipase recombination target) site, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP site, mouse Ube3c genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data