RBRC06270

Information1

Image
BRC No.RBRC06270
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tgm3<tm1a(KOMP)Wtsi>/B12
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tgm3transglutaminase 3, E polypeptide2Tgm3<tm1a(KOMP)Wtsi>targeted mutation 1a, Wellcome Trust Sanger Institute

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)2En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal2

Information2

Donor DNAyeast Frt sites, En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, Bacteriophage P1 loxP sites, human beta actin promoter, E. coli Neomycin resistance gene, mouse Tgm3 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT should acknowledge the KOMP, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data