Strain Data Sheet



BRC No.RBRC06249
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ret<tm1Mat>
Former Common nameB6-Ret YF KI, B6-Ret/YF KI
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Masahide Takahashi, Nagoya University Graduate School of Medicine. ES cells derived from 129 were used for generation of the mice. The mutant mice were backcrossed to C57BL/6 over 16 times.
Strain descriptionRet Y1062F variant knock-in mice having two amino acid replacement (KLY<1062> to KIF<1062>) within exon 19 and a floxed PGK-neo cassette inserted into intron 19. Homozygous mutant mice are born normally, but exhibit megacolon and deafness and die by day 27 after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesMol. Cell Biol., 24, 8026-8036 (2004). 15340065
Proc. Natl. Acad. Sci. USA, 107(29), 13051-13056 (2010). 20616061

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Retret proto-oncogene6Ret<tm1Mat>targeted mutation 1, Masahide Takahashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAphage P1 LoxP sites, Mus musclus PGK promoter, Escherichia coli Neomycin resistance gene, mouse Ret genomic DNA
Research applicationCre/loxP system
Mouse Models for Human Disease
Sensorineural Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasashi Kato (Chubu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data