RBRC06248

Information1

Image
BRC No.RBRC06248
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Brd1<tm2Hko>
Former Common nameBrd1 cKO, B6-Brd1 floxed
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Atsushi Iwama, Graduate School of Medicine, Chiba University and Akihiko Koseki, RIKEN Research Center for Allergy and Immunology in 2009. The mutant mice were backcrossed to C57BL/6N over 7 times.
Strain descriptionBrd1 gene floxed mice. Exon 2 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
ReferencesBlood, 118, 2443-2453 (2011). 21753189

Health Report

Examination Date / Room / Rack2014/09/08Room:2-検Rack:N
2014/07/07Room:2-検Rack:N

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Brd1bromodomain containing 115Brd1<tm2Hko>targeted mutation 2, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Information2

Donor DNAphage P1 loxP sites, Sacchoromces cerevisiae FRT site, mouse Brd1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。(will be announced).
DepositorAtsushi Iwama (Chiba University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data