RBRC06246

Information1

Image
BRC No.RBRC06246
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gab2<tm4.1Thir>
Former Common nameGab2 Y441F/Y465F/Y573F KI, Gab2 deltaPI3K KI, B6-Gab2/dPI3K KI
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Keigo Nishida, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. A floxed neo cassette was removed by crossing with B6-CAG-cre mice. C57BL/6 and 129 mixed background.
Strain descriptionGab2 mutant gene knock-in mice. A cDNA encoding 89-665 amino acids was inserted into exon 2 of Gab2 gene. The cDNAs encoded wild-type (WT) (RBRC06244), Y603F/Y632F mutant abolishing the SHP2-binding domain (RBRC06245), Y441F/Y465F/Y573F mutant abolishing PI3K-binding domain (RBRC06246) and Y441F/Y465F/Y573F/Y603F/Y632F mutant abolishing the SHP2 and PI3K-binding domains (RBRC06247) mutant, respectively.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Immunol., 187, 932-941 (2011). 21653832

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gab2growth factor receptor bound protein 2-associated protein 27Gab2<tm4.1Thir>targeted mutation 4.1, Toshio Hirano

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)7GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gab2mouse Gab2 Y441F/Y465F/Y573F cDNA encoding amino acid 89-6657Gab2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Information2

Donor DNAmouse Gab2 genomic DNA, mouse Gab2 Y441F/Y465F/Y573F cDNA encoding amino acid 89-665, bovine growth hormone polyadenylation signal, phage P1 loxP
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 187, 932-941 (2011).
DepositorKeigo Nishida (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data