Strain Data Sheet


Strain Information

BRC No.RBRC06240
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Hmgb1<tm1Ttg>
Former Common nameHmgb1 cKO, B6-Hmgb1 floxed
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Strain developmentDeveloped by Hideyuki Yanai and Tadatsugu Taniguchi, Institute of Industrial Science, University of Tokyo and Nobuaki Yoshida, Institute of Medical Science, University of Tokyo in 2011. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6.
Strain descriptionHmgb1 gene floxed mice. Exons 2 to 4 were flanked by loxP sites. Homozygous mutant mice are viable, but show weak fertility.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 110, 20699-20704 (2013). 24302768

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hmgb1high mobility group box 15Hmgb1<tm1Ttg>targeted mutation 1, Tadatsugu Taniguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Ordering Information

Donor DNAPhage P1 loxP sites, mouse Hmgb1 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTadatsugu Taniguchi (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Jun 2014

BRC mice in Publications

Ahmed C, Greve HJ, Garza-Lombo C, Malley JA, Johnson JA Jr, Oblak AL, Block ML.
Peripheral HMGB1 is linked to O3 pathology of disease-associated astrocytes and amyloid.
Alzheimers Dement 20(5) 3551-3566(2024) 38624088

Lu J, Yue Y, Xiong S.
Extracellular HMGB1 augments macrophage inflammation by facilitating the endosomal accumulation of ALD-DNA via TLR2/4-mediated endocytosis.
Biochim Biophys Acta Mol Basis Dis 1867(10) 166184(2021) 34087422