Strain Data Sheet


Strain Information

BRC No.RBRC06239
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Siglec1<tm1(cre)Mtka>
Former Common nameCD169-Cre, B6-Siglec1/Cre KI
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Strain developmentDeveloped by Dr. Masato Tanaka, Tokyo University of Pharmacy and Life Sciences, School of Life Science. B6JN/1 ES cells were used. C57BL/6 background.
Strain descriptionCD169 (Siglec1) cre knock-in mice. iCre recombinase is expressed from CD169 locus.
Colony maintenanceHomozygote x Homozygote [or crossing to C57BL/6JJcl]
ReferencesJ. Am. Soc. Nephrol., 26, 896-906 (2015). 25266072
Genesis, 32(1):19-26 (2002). 11835670
Nat. Commun., 6:7802 (2015). 26193821

Health Report

Examination Date / Room / Rack2024/06/10Room:3-2Rack:C


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Siglec1sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(cre)Mtka>targeted mutation 1, Masato Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iCrephage P1 codon improved Cre (iCre)2iCre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
polyoma virus polyA2

Ordering Information

Donor DNAPhage P1 improved Cre recombinase, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyoma virus polyA, mouse Siglec1 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Am. Soc. Nephrol., 26, 896-906 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER, and then include them as co-authors in any publications resulted by the use of the BIOLOGICAL RESOURCE.
DepositorMasato Tanaka (Tokyo University of Pharmacy and Life Sciences)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Camara A, Cordeiro OG, Alloush F, Sponsel J, Chypre M, Onder L, Asano K, Tanaka M, Yagita H, Ludewig B, Flacher V, Mueller CG.
Lymph Node Mesenchymal and Endothelial Stromal Cells Cooperate via the RANK-RANKL Cytokine Axis to Shape the Sinusoidal Macrophage Niche.
Immunity 50(6) 1467-1481.e6(2019) 31201093

Camara A, Lavanant AC, Abe J, Desforges HL, Alexandre YO, Girardi E, Igamberdieva Z, Asano K, Tanaka M, Hehlgans T, Pfeffer K, Pfeffer S, Mueller SN, Stein JV, Mueller CG.
CD169+ macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling.
Proc Natl Acad Sci U S A 119(3) (2022) 35031565