RBRC06238

Information1

Image
BRC No.RBRC06238
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Capn6<tm1Hiso>
Former Common nameICR-Capn6 KO
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuo Tonami and Hiroki Kurihara, Graduate School of Medicine, The Tokyo University in 2004. Maintained by Tokyo Metropolitan Institute of Medical Science. G4 ES cells were used. The mutant mice were crossed to C57BL/6 (RBRC06237) and ICR (RBRC06238).
Strain descriptionCapn6 gene knockout mice. Exon 2 of Capn6 was replaced with a nls-lacZ/PGK-neo cassette. Capn6 deficient mice (homozygous females and hemizygous male) were viable, but display precocious development of embryonic skeletal muscle and promote the differentiation of skeletal muscle progenitors cells. X linked. C57BL/6 background (RBRC06237) and ICR mixed background (RBRC06238).
Colony maintenanceHomozygote (female) x Hemizygote (male) [or Crossing to Crlj:CD1(ICR)]
ReferencesPLoS Genet., 9:e1003668 (p1-11). 23935533

Health Report

Examination Date / Room / Rack2015/04/06Room:3-5Rack:B
2015/02/02Room:3-5Rack:B
2014/12/01Room:3-5Rack:B
2014/10/06Room:3-5Rack:B
2014/08/04Room:3-5Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Capn6calpain 6XCapn6<tm1Hiso>targeted mutation 1, Hiroyuki Sorimachi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)Xnls

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalX

Information2

Donor DNAphage P1 mutant loxP sequence, SV40 nls (nuclear localization signal), E. coli lacZ gene, SV40 PolyA signal, Mouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, bovine growth hormone 1 poly A, Mouse Capn6 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet. 2013 9(8):e1003668.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
In presenting the research results obtained by use of the BIOLOGICAL RESOURCE for the first time in a form of international publications, it is required that the RECIPIENT contacts the DEPOSITOR for agreement and that the DEPOSITOR and its colleagues (Kazuo Tonami, Hiroki Kurihara, and Hiroyuki Sorimachi) are included as co-authors.
DepositorHiroyuki Sorimachi (Tokyo Metropolitan Institute of Medical Science)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data