Strain Data Sheet

RBRC06232

Strain Information

Image
BRC No.RBRC06232
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Prl<tm1Hmn>
Former Common namePRL-KO, B6-Prl KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Nelson Horseman, University of Cincinnati and Minoru Tanaka, Mie University School of Medicine in 1997. D3 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 20 times.
Strain descriptionPrl (prolaction) gene knockout mice. A PGK-neo cassette was inserted into the exon 4 of Prl gene. Homozygous mutant females are infertile due to an implantation defect. Homozygous mutant males are fertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Defective mammopoiesis, but normal hematopoiesis, in mice with a targeted disruption of the prolactin gene.
Horseman N D, Zhao W, Montecino-Rodriguez E, Tanaka M, Nakashima K, Engle S J, Smith F, Markoff E, Dorshkind K
EMBO J., 16, 6926-6935 (1997). 9384572

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Prlprolactin13Prltargeted mutation 1, Nelson D Horseman
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal branching of the mammary ductal tree(MP:0000662)

  • abnormal estrous cycle(MP:0001927)

  • abnormal gonadotroph morphology(MP:0008325)

  • abnormal mammary gland development(MP:0000628)

  • abnormal mammary gland morphology(MP:0000627)
    • more 28 phenotypes
  • abnormal pituitary gland morphology(MP:0000633)

  • abnormal pituitary secretion(MP:0001746)

  • behavior/neurological phenotype(MP:0005386)

  • decreased circulating estradiol level(MP:0005181)

  • decreased circulating estrogen level(MP:0003371)

  • decreased circulating luteinizing hormone level(MP:0002773)

  • decreased circulating prolactin level(MP:0005121)

  • decreased dopamine level(MP:0005643)

  • decreased follicle stimulating hormone level(MP:0005130)

  • decreased lactotroph cell number(MP:0008332)

  • decreased liver weight(MP:0003402)

  • decreased luteinizing hormone level(MP:0005132)

  • decreased prolactin level(MP:0005138)

  • decreased seminal vesicle weight(MP:0004910)

  • endocrine/exocrine gland phenotype(MP:0005379)

  • enlarged pituitary gland(MP:0000636)

  • failure of embryo implantation(MP:0001728)

  • female infertility(MP:0001926)

  • immune system phenotype(MP:0005387)

  • increased pituitary adenoma incidence(MP:0002041)

  • increased pituitary gland weight(MP:0008939)

  • pituitary gland hyperplasia(MP:0000635)

  • pituitary gland hypertrophy(MP:0013336)

  • prolonged estrous cycle(MP:0009006)

  • prolonged estrus(MP:0009017)

  • prolonged proestrus(MP:0009014)

  • short estrous cycle(MP:0009007)

  • small prostate gland ventral lobe(MP:0000661)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter, Mouse Prl genomic DNA
    Research applicationDevelopmental Biology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 16, 6926-6935 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorMInoru Tanaka (Nippon Veterinary and Animal Science University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data