Strain Data Sheet


Strain Information

BRC No.RBRC06232
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Prl<tm1Hmn>
Former Common namePRL-KO, B6-Prl KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Strain developmentDeveloped by Nelson Horseman, University of Cincinnati and Minoru Tanaka, Mie University School of Medicine in 1997. D3 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 20 times.
Strain descriptionPrl (prolaction) gene knockout mice. A PGK-neo cassette was inserted into the exon 4 of Prl gene. Homozygous mutant females are infertile due to an implantation defect. Homozygous mutant males are fertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesEMBO J., 16, 6926-6935 (1997). 9384572

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prlprolactin13Prl<tm1Hmn>targeted mutation 1, Nelson D Horseman

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter, Mouse Prl genomic DNA
Research applicationDevelopmental Biology Research
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 16, 6926-6935 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorMInoru Tanaka (Nippon Veterinary and Animal Science University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data