RBRC06226

Information1

Image
BRC No.RBRC06226
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Bcl11b<tm2.1Jpk>
Former Common nameB6-Bcl11b floxed
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Ryo Kominami, Graduate School of Medical and Dental Science and Kenji Sakimura, Brain Research Institute, Niigata University. RENKA ES cells derived from C57BL/6NCrlCrlj were used for the generation of floxed mice. Neo cassette was removed by crossing with B6-CAG-FLPe mice (RBRC01824). C57BL/6 background (RBRC06226), BALB/c background (RBRC06229).
Strain descriptionBcl11b floxed mice. Exon 2 was flanked by loxP sites. C57BL/6 background (RBRC06226), BALB/c background (RBRC06229).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesCancer Sci., 104. 1009-1016 (2013). 23663453

Health Report

Examination Date / Room / Rack2018/06/04Room:3-3Rack:D
2018/04/02Room:3-3Rack:D
2018/02/05Room:3-3Rack:D
2017/12/04Room:3-3Rack:D
2017/10/24Room:3-3Rack:D
2017/10/02Room:3-3Rack:D
2017/08/21Room:3-3Rack:D
2017/06/05Room:3-3Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bcl11bB cell leukemia/lymphoma 11B12Bcl11b<tm2.1Jpk>targeted mutation 2.1, Japan Kominami

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Information2

Donor DNAP1 phage loxP sites, Saccharomyces cerevisiae FRT site, mouse Bcl11b genomic DNA
Research applicationCancer Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorRyo Kominami (Niigata University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data