RBRC06222

Information1

Image
BRC No.RBRC06222
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Slc39a14<tm1Thir>
Former Common nameZIP14 KO, B6- Slc39a14 KO
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Toshiyuki Fukuda, RIKEN Center for Allergy and Immunology in 2011. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. C57BL/6 and 129 mixed background.
Strain descriptionZip14 (Slc39a14) gene knockout mice. Exons 5 through 8 were replaced with a neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesPLoS One. 6(3):e18059 (2011) 21445361

Health Report

Examination Date / Room / Rack2015/07/27Room:4-ARack:F
2015/05/25Room:4-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Slc39a14solute carrier family 39 (zinc transporter), member 1414Slc39a14<tm1Thir>targeted mutation 1, Toshio Hirano

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase (Pgk) promoter, E. coli Neomycin resistance gene, mouse Slc39a13 genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2011 6(3):e18059.
DepositorDrs. Shigeo Koyasu and Toshiyuki Fukada (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data