RBRC06218

Information1

Image
BRC No.RBRC06218
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Susd3<tm1(KOMP)Vlcg>
Former Common nameSusd3 KO, B6-Susd3 KO
H-2 Haplotype
ES Cell lineVGB6 [C57BL/6NTac]
Background strainC57BL/6NTac
Appearance
Strain developmentDeveloped by Yoshihiro Omori and Takahisa Furukawa, Institute for Protein Research, Osaka University. The original allele and mouse were on C57BL/6 background and from the Knockout Mouse Project (KOMP) Repository.
Strain descriptionSusd3, sushi domain containing 3 gene knockout mice. A 17004 bp region between 49,343,498-49,326,495 positions of Chromosome 13 was deleted by the insertion of ZEN-Ub1 cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
References

Health Report

Examination Date / Room / Rack2014/09/08Room:2-検Rack:E
2014/07/07Room:2-検Rack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Susd3sushi domain containing 313Susd3<tm1(KOMP)Vlcg>targeted mutation 1, Velocigene

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pgk1mouse PGK polyA signal13Pgk1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13human ubiquitin C promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal13

Information2

Donor DNAVelocigene cassette ZEN-Ub1(E. coli Beta-galactosidase, SV40 polyA, phage P1 loxP sites, human ubiquitin C promoter, E. coli neoR gene, mouse PGK polyA), mouse Susd3 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data