RBRC06212

Information1

Image
BRC No.RBRC06212
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Bdnf<tm1.2Hko>
Former Common nameBDNF Delta FRT/+ (GWAS005), B6-Bdnf floxed
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. B6JN/1 ES cells derived from C57BL/6 were used for the generation of floxed mice. Neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain descriptionBDNF (Bdnf) gene floxed mice. Exon 5 was flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack2020/06/01Room:3-ARack:B
2020/02/17Room:3-ARack:B
2019/12/16Room:3-ARack:B
2019/10/21Room:3-ARack:B
2019/08/26Room:3-ARack:B
2019/06/17Room:3-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Bdnfbrain derived neurotrophic factor2Bdnf<tm1.2Hko>targeted mutation 1.2, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Bdnf genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data