Strain Data Sheet
RBRC06211
Strain Information | |
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| Image | |
| BRC No. | RBRC06211 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129-Piga<tm1Tak> |
| Former Common name | Piga floxed, B6-Piga floxed |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Junji Takeda and Taroh Kinoshita, Research Institute for Microbial Diseases, Osaka University in 1997. R1 ES cells derived were used for the generation of floxed mice. The mutant mice were backcrossed to C57BL/6J over 10 times. |
| Strain description | Piga gene floxed mice. A loxP site was inserted between exons 5 and 6, and a loxP flanked Tk-neo cassette was inserted after the exon 5. |
| Colony maintenance | Heterozygote, Hemizygote x Wild-type [C57BL/6JJcl] |
| References | Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development. M Tarutani, S Itami, M Okabe, M Ikawa, T Tezuka, K Yoshikawa, T Kinoshita, J Takeda Proc. Natl. Acad. Sci. USA, 94, 7400-7405 (1997). 9207103 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Piga MGI:99461 | phosphatidylinositol glycan anchor biosynthesis, class A | X | Piga<tm1Tak> MGI:2386120 | targeted mutation 1, Junji Takeda | |||
| loxP | phage P1 loxP | X | loxP | ||||
| loxP | phage P1 loxP | X | loxP | ||||
| loxP | phage P1 loxP | X | loxP | ||||
| neo | neomycin resistance gene (E. coli) | X | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | more 3 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Piga genomic DNA |
| Research application | Cre/loxP system Hematological Research Mouse Models for Human Disease |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Taroh Kinoshita (The University of Osaka) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Kandasamy LC, Tsukamoto M, Banov V, Tsetsegee S, Nagasawa Y, Kato M, Matsumoto N, Takeda J, Itohara S, Ogawa S, Young LJ, Zhang Q. Limb-clasping, cognitive deficit and increased vulnerability to kainic acid-induced seizures in neuronal glycosylphosphatidylinositol deficiency mouse models. Hum Mol Genet 30(9) 758-770(2021) 33607654 |
Lukacs M, Blizzard LE, Stottmann RW. CNS glycosylphosphatidylinositol deficiency results in delayed white matter development, ataxia and premature death in a novel mouse model. Hum Mol Genet 29(7) 1205-1217(2020) 32179897 |