RBRC06209

Information1

Image
BRC No.RBRC06209
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1(Dte/3xHA)Hmd>
Former Common nameROSA26-3HA Dte KI
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells were used. C57BL/6 and 129 mixed background.
Strain descriptionDte-3xHAtag ROSA26 conditional knock in mice. PGK-neo cassette with SV40 ployA and Dte (Dand5)-3xHA were inserted into the Gt(ROSA)26Sor. The Dte-3xHA expression can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesDev Biol.,15;395(2), 331-341 (2014). 25224222

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Dte/3xHA)Hmd>targeted mutation 1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dand5DAN domain family, member 56Dand5<tm1Hmd>targeted mutation 1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 virus polyA signal sequence6

Information2

Donor DNAAdenovirus splicing acceptor (SA), mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, SV40 virus polyA signal sequence, Influenza virus HA tag, phage P1 loxP sites, mouse Dand5 (Cerberus like 2) gene
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data