BRC No.RBRC06208
SpeciesMus musculus
Strain nameB6C3-Tg(Cerl2-sGFP*d3*dInt)25Hmd
Former Common nameCerl2(Cerl2-sGFP-Delta 3'-Delta int)BAC(25)
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and C3H mixed background.
Strain descriptionBAC Cerl2-sGFP (delta 3’,delta intron) transgenic mice. The mice were generated by using a BAC clone containing the mouse Cerl2 gene, PR23-421H21. The sGFP (a 300-bp sequence coding for a portion of EGFP) gene was inserted into the exon 2 of in the Cerl2 BAC clone. The 3’-UTR of Cerl2 was replaced by the polyA sequence (250 bp) of SV40, and intron 1of Cerl2 had been excised.
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6C3F1]
ReferencesNat Commun. 2012;3:1322. doi: 10.1038/ncomms2319. 23271656

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFPmouse Dand5 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 virus polyA signal sequenceUN


Donor DNAmouse Cerberus like 2 gene, Aequorea victoria a portion of EGFP gene, SV40 virus polyA signal sequence
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data