RBRC06203

Information1

Image
BRC No.RBRC06203
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pitx2<tm1Hmd>
Former Common namePitx2 A-17 flox, B6-Pitx2 floxed
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Hidetaka Shiratori and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2001. G4 ES cells were used. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and 129 mixed background.
Strain descriptionPitx2 gene floxed mice. A loxP site was inserted in the intron 5, and a neo cassette with a 3’ loxP site was inserted downstream of intron 5. Homozygous mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesDev. Dyn., 240, 195-203 (2011). 21089073

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pitx2paired-like homeodomain transcription factor 23Pitx2<tm1.1Mbf>targeted mutation 1.1, Milena B Furtado

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, mouse PGK1 promoter, E.coli neo gene, mouse PGK1 polyA signal sequence, mouse Pitx2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data