RBRC06199

Information1

Image
BRC No.RBRC06199
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(NDE-hsp-GCaMP2,lacZ)1Hmd
Former Common nameNDE-GCaMP2-IZ(1), B6-NDE-GCaMP2/lacZ Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Satoko Yoshiba and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. The NDE-hsp- GCaMP2,lacZ transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6, 129 and C3H mixed background.
Strain descriptionThis transgenic mouse expresses GCaMP2 and lacZ genes on both sides of the node under the control of the mouse node-specific enhancer.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesNat. Biotechnol., 19, 137-141 (2001). 11175727
Science, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GCaMP2RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP2mouse node-notochord enhancer, mouse Hsp68 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAmouse node-notochord enhancer, mouse Hsp68 promoter, GCaMP2(RSET tag, chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyA signal
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data