RBRC06197

Information1

Image
BRC No.RBRC06197
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Cckar<tm1Kym>
Former Common nameCCKAR KO, B6-Cckar KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Soichi Takiguchi and Akihiro Funakoshi, National Kyusu Cancer Center, Tetsuo Noda, The Cancer Institute, Japanese Foundation for Cancer Research and Kyoko Miyasaka, Tokyo Metropolitan Institute of Gerontology. J1 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionCckar gene knockout mice. Part of exon 1 and all of exon 2 were replaced with a NLS-lacZ neo cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
ReferencesPancres, 24, 276-283 (2002). 11893936

Health Report

Examination Date / Room / Rack2014/06/16Room:3-ARack:H
2014/04/21Room:3-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cckarcholecystokinin A receptor5Cckar<tm1Kym>targeted mutation 1, Kyoko Miyasaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)5nls

Information2

Donor DNASV40 Large T antigen nuclear localisation signal (NLS), E. coli Beta-Galactosidase gene (LacZ gene), Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Cckar genomic DNA
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Pancres, 24, 276-283 (2002).
DepositorKyoko Miyasaka (Tokyo Kasei University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data