RBRC06193

Information1

Image
BRC No.RBRC06193
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Pax7-eGFP)13Nah
Former Common namePaxG13, B6-Pax7-eGFP Tg#13
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Naohiro Hashimoto, National Center for Geriatrics and Gerontology in 2004. The mutant mice were backcrossed to C57BL/6.
Strain descriptionPax7-GFP BAC transgenic mice. The GFP cDNA was inserted into the exon 1 of Pax7 BAC gene. Expresion of the transgene mostly matches that of the endogenous Pax7 in the nervous system, but, limited to part of anti-Pax7 positive cells in the muscle satellite cells.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack2014/09/08Room:2-検Rack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
CmChloramphenicol resistantce gene (E. coli)UNCm

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFPmouse Pax7 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAmouse Pax7 genomic appx.150 kb BAC clone, Jellyfish GFP cDNA, SV40 polyA signal, E. coli Chloramphenicol resistance gene, (E. coli lacZ), Phage P1 loxP site derived from BAC vector
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorNaohiro Hashimoto (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data