RBRC06190

Information1

Image
BRC No.RBRC06190
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameD2;129-Myod1<tm1Jae>
Former Common nameMyoD-KO, D2-Myod1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Rudolf Jaenisch, Whitehead Institute and Department of Biology Massachusetts Institute of Technology Cambridge. J1 ES cells were used. The mutant mice were maintained by Naohiro Hashimoto, National Center for Geriatrics and Gerontology. 129 and DBA/2 mixed background.
Strain descriptionMyoD1 gene knockout mice. The Myod1 promoter, exon 1 and half of intron 1 were replaced with a a neomycin cassette.
Colony maintenanceHomozygote x Homozygote
ReferencesCell, 71, 383-390 (1992). 1330322

Health Report

Examination Date / Room / Rack2018/12/17Room:3-BRack:H
2018/10/15Room:3-BRack:H
2018/08/20Room:3-BRack:H
2018/06/18Room:3-BRack:H
2018/04/16Room:3-BRack:H
2018/02/19Room:3-BRack:H
2017/12/18Room:3-BRack:H
2017/10/16Room:3-BRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Myod1myogenic differentiation 17Myod1<tm1Jae>targeted mutation 1, Rudolf Jaenisch

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (Klebsiella pneumoniae)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Myod1 genomic DNA, Klebsiella pneumoniae neomycin resistance gene, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationMouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 71, 383-390 (1992).
DepositorNaohiro Hashimoto (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data